中文摘要：生物技术通过引入转基因食品以缓解粮食不足的问题。市场上转基因食品的存在和食品标签信息的缺乏，表明了产品交易、产地认证和食品质量需要开发强有力的工具来检测食品中的转基因生物。本文对番茄加工食品的DNA完整性进行了评价。番茄酱的制备需要评价添加剂对番茄DNA基因组的完整性、质量和扩增性的影响，进而进行转基因检测。作者将新鲜番茄样品作阳性对照分析。accD、LAT52、和PG扩增表明存在转基因番茄果实。影响转基因检测的最重要因素是成分、酸添加剂和贮存时间。研究结果表明，DNA抽提物的质量和完整性对于开发检测转基因和食品追溯性的分子技术是非常重要的。
外文摘要：Biotechnology helps to alleviate problems of insufficient food by introducing transgenic foodstuff. The existence of transgenic food in the market and the lack of information in food labeling indicating the product's action, certification of origin, and the quality of foodstuff have required the development of powerful tools to detect the transgenic organisms in food. In this study the DNA's integrity processed tomato food was evaluated. Preparation of tomato puree and ketchup were required to evaluate the influence of additives on the integrity, quality, and amplificability of genomic DNA of tomato to detect transgenic genes. Samples of fresh tomato were analyzed as positive control. The accD, LAT52, and PG amplicons indicated the presence of transgenic tomato fruits. The ingredients or acid additives and storage time were the most important factors that affected the detection of transgenes. The results confirmed that quality and integrity of the DNA extracts are very important to develop the molecular techniques for the detection of transgenes and food traceability.
外文关键词：DNA integrity; transgenes; processed food; traceability; tomato
作者：Villa-Morales, A.; Minor-Perez, H.; Lopez, R. Aguilar; 等.
作者单位：Inst Politecn Nacl
期刊名称：REVISTA MEXICANA DE INGENIERIA QUIMICA
期刊影响因子：0.924
出版年份：2017
出版刊次：8
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