中文摘要：本研究旨在建立一种用高效液相色谱-串联质谱(LC-MS/MS) 在正负离子模式下检测并量化红辣椒粉样品中多种霉菌毒素如黄曲霉毒素、赭曲霉素A及玉米赤霉烯酮的快速、简单、经济、有效、稳定而安全(QuEChERS)的提取和净化的方法。结果表明用本LC-MS/MS结合QuEChERS法检测多种霉菌毒素对控制红辣椒粉的质量及安全可能有用。
外文摘要：In this study, we aimed to establish a method for determination of multiple mycotoxins such as afiatoxins, ochratoxin A, and zearalenone in red pepper powder samples based on the quick, easy, cheap, effective, rugged, and safe (QuEChERS) approach for extraction and cleanup, with detection and quantification by high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in both positive- and negative-ion modes. The developed LC-MS/MS analytical method was compared with an enzyme-linked immunosorbent assay (ELISA) to improve the reliability of our developed method. The linearity, precision, and accuracy were validated for the LC-MS/MS methods. The results obtained with the LC-MS/MS were linear, with a correlation coefficient (R-2) of >0.998. The limits of detection and quantification for mycotoxins were 0.07 to 0.71 mu g/kg and 0.20 to 1.81 mu g/kg, respectively. Intra- and interday precision tests (expressed as the relative standard deviation) for each analyte were 1.58 to 5.97% and 0.97 to 9.01%, respectively. Average recoveries were 85.70 to 94.99%. The validation results for the ELISA were linear (R-2 > 0.995), and recoveries were 77.13 to 93.93%. Both analytical methods were applied to determine the presence of mycotoxins in commercial red pepper powder samples from South Korea. Four of the total 56 samples were contaminated with afiatoxins, and 6 samples were contaminated with ochratoxin A; these results were consistent for the two methods (P > 0.05). Therefore, our developed LC-MS/MS with QuEChERS approach for determination of multiple mycotoxins may be useful for controlling the quality and safety of red pepper powder.
外文关键词：Enzyme-linked immunosorbent assay；Mycotoxin；Quick, easy, cheap, effective, rugged and safe approach；Red pepper powder；Ultra-performance liquid chromatography coupled with tandem mass spectrometry
作者：Kim, S；Lee, S；Nam, TG；Seo, D；Yoo, M；AF Kim, Sunyoung；Lee, Sanghee；Nam, Tae-Gyu；Seo, Dongwon；Yoo, Miyoung
作者单位：Korea Food Res Inst
期刊名称：JOURNAL OF FOOD PROTECTION
期刊影响因子：1.609
出版年份：2017
出版刊次：8
点击下载：对一种新开发的液相色谱串联质谱法与酶联免疫吸附测定两种方法检测红辣椒粉中多种霉菌毒素进行比较