中文摘要：黄曲霉毒素B1（AFB1）是污染食品及饲料的主要真菌毒素之一，引起氧化应激和肝毒性。据报道姜酚具有抗氧化及保肝活性。本研究探索了富含酚的生姜提取物(GE)对AFB1诱导的氧化应激及肝毒性的保护作用。结果证实了GE具有保护AFB1诱导的氧化应激及肝毒性的作用。
外文摘要：Aflatoxin B-1 (AFB(1)) is one of the predominant mycotoxin contaminant in food and feed, causing oxidative stress and hepatotoxicity. Ginger phenolics have been reported for its antioxidant potential and hepatoprotective activity. The present study investigated the protective effects of phenolics rich ginger extract (GE) against AFB(1) induced oxidative stress and hepatotoxicity, in vitro and in vivo. The phenolic acid profiles of GE showed 6-gingerol and 6-shogaol as predominant components. Pretreatment of HepG2 cells with GE significantly inhibited the production of intracellular reactive oxygen species (ROS), DNA strand break, and cytotoxicity induced by AFB(1). A comparable effect was observed in in vivo. Male Wistar rats were orally treated with GE (100 and 250 mg/kg) daily, with the administration of AFB(1) (200 mg/kg) every alternative day for 28 days. Treatment with GE significantly reduced AFB(1) induced toxicity on the serum markers of liver damage. In addition, GE also showed significant hepatoprotective effect by reducing the lipid peroxidation and by enhancing the antioxidant enzymes activities. These results combined with liver histopathological observations indicated that GE has potential protective effect against AFB(1) induced hepatotoxicity. Additionally, administration of GE up-regulated Nrf2/HO-1 pathway, which further proved the efficiency of GE to inhibit AFB(1) induced hepatotoxicity.
外文关键词：Aflatoxin B-1；Hepatotoxicity；Ginger phenolics；Oxidative stress；Nrf(2)
作者：Vipin, AV；Rao, KR；Kurrey, NK；Appaiah, KAA；Venkateswaran, G；AF Vipin, A. V.；Rao, Raksha K.；Kurrey, Nawneet Kumar；Appaiah, Anu K. A.；Venkateswaran, G.
作者单位：CSIR
期刊名称：BIOMEDICINE & PHARMACOTHERAPY
期刊影响因子：2.326
出版年份：2017
出版刊次：-
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