中文摘要：由于转基因生物越来越受到人们的重视，因而对高通量检测方法的需求越来越大。本文利用一种高通量、低成本的多重串联PCR对14个目标转基因玉米进行了筛选试验，并利用SYBR Green I方法对7个转基因共同筛选要素、6个转基因玉米品种和玉米参考基因进行了定量。研究结果表明，多重串联PCR技术可有效地用于食品和饲料中转基因玉米的筛选。
外文摘要：Genetically modified organisms (GMO) are increasingly gaining acceptance, which has led to an increasing demand for high-throughput methods for their detection. This study describes development of a high-throughput and low-cost multiplex tandem PCR (MT-PCR) assay for the screening of transgenic maize of 14 targets, containing 7 genetically modified (GM) common screening elements, 6 transgenic maize events and a maize reference gene, using SYBR Green I for quantification. The 14-target high-throughput multiplex tandem PCR assay successfully amplified products from single-event samples with a GM maize DNA amount less to 0.001 ng, and displayed high specificity. This assay can efficiently be used for screening GM maize in foods and feeds.
外文关键词：Multiplex tandem PCR; Genetically modified maize; Screening
作者：Wei, Shuang; Wang, Chenguang; Zhu, Pengyu; 等.
作者单位：Jinan Univ
期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY
期刊影响因子：2.329
出版年份：2018
出版刊次：1
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