中文摘要：自1997年开始，中国大面积种植的转基因抗虫棉可控制棉铃虫从而增加产量、减少杀虫剂的使用，但是棉铃虫对转基因抗虫棉的抗性演化是一个新的挑战。作者之前研发了一种新型的转基因抗虫棉花Vip3AcAa，其具有更广泛的杀虫谱。本文主要研究了Vip3AcAa和Cry1Ac对三个棉铃虫植株的交叉抗性和相互作用，其中两株具有Cry1Ac抗性，以判定Vip3AcAa是否可作为与Cry1Ac毒素共价的转基因棉花候选。研发发现，Cry1Ac毒素没有影响Cry1Ac抗性菌株对vip3acaa的敏感性。在转双价基因（Vip3AcAa+Cry1Ac）棉花中，抗Cry1Ac棉铃虫幼虫的死亡率明显高于取食非转基因棉花和的转Cry1Ac基因棉花的死亡率。研究结果表明，在中国Vip3AcAa蛋白是抗病虫害管理战略的一个很好选择。
外文摘要：Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (Helicoverpa armigera) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by H. armigera remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three H. armigera strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric vip3AcAa gene and the cry1Ac gene were introduced into cotton, mortality rates of Cry1Ac resistant H. armigera larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a "pyramid" strategy for pest resistance management in China.
外文关键词：Bacillus thuringiensis; Vegetative insecticidal protein (Vip3AcAa); Helicoverpa armigera; Cross resistance; Transgenic cotton
作者：Chen, Wen-bo; Lu, Guo-qing; Cheng, Hong-mei; 等.
作者单位：Chinese Acad Agr Sci
期刊名称：TRANSGENIC RESEARCH
期刊影响因子：2.341
出版年份：2017
出版刊次：10
点击下载：转双价基因（Vip3AcAa+Cry1Ac）棉花对抗Cry1Ac棉铃虫的有效保护研究