中文摘要：紫肉甘薯中含有的花青素对人体健康有益。叶色（Lc）基因是调控花青素生物合成的转录因子。本文采用高效液相色谱-串联质谱技术研究了野生紫肉甘薯和三种转LC基因紫肉甘薯的花青素。作者测量了野生紫肉甘薯和转LC基因紫肉甘薯的抗氧化能力，包括还原能力活性、DPPH自由基活性、羟自由基清除活性、亚油酸氧化抑制活性、ABTS自由基清除活性、氧自由基吸收能力活性等。研究发现，三种转LC基因紫肉甘薯中的花青素含量比野生紫肉甘薯中的花青素含量增加了1.5-1.9倍；在野生紫肉甘薯中发现了17中花青素，而在转LC基因紫肉甘薯发现了19中花青素。野生紫肉甘薯和三种转LC基因紫肉甘薯中花青素的抗氧化活性没有显著差异。研究结果表明，转LC基因紫肉甘薯花青素的总含量增加，花青素组成发生了显著变化。在具有强大抗氧化活性的转LC基因紫肉甘薯中可获得这种新颖的高含量花青素，这可能为提供有益于人类健康的产品。
外文摘要：BACKGROUND Anthocyanins in purple-fleshed sweet potato (PSP) are beneficial to human health. The leaf color (Lc) gene is a transcription factor involved in regulating anthocyanin biosynthesis. The anthocyanin profiles of wild-type PSP of Ayamurasaki and its three Lc-transgenic lines were investigated by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). In vitro antioxidant activities of wild-type and Lc-transgenic lines, including reducing power activity, DPPH radical scavenging activity, hydroxyl radical scavenging activity, linoleic acid autoxidation inhibition activity, ABTS free radical scavenging activity and oxygen radical absorbance capacity activity, were measured. RESULTSThe results showed that the total anthocyanin contents increased 1.5-1.9 times in three transgenic lines compared with that in wild-type PSP. Seventeen anthocyanins were found in wild-type PSP, while 19 in Lc-transgenic lines including cyanidin-based, peonidin-based and pelargonidin-based anthocyanins. Three pelargonidin-based anthocyanins were detected in three Lc-transgenic lines. Among them, the relative contents of cyanidin-based and pelargonidin-based anthocyanins increased 1.9-2.0 and 3.4-4.5 times respectively, while peonidin-based anthocyanins decreased 1.8-1.9 times in Lc-transgenic lines, compared with wild-type PSP. PSP from wild-type Ayamurasaki and three Lc-transgenic lines exhibited potent antioxidant activities, whereas there was no distinct difference among them. CONCLUSIONThe transgene Lc significantly increased the content of total anthocyanins and remarkably changed the anthocyanin profiles in Ayamurasaki. Such novel and high content of anthocyanins obtained in the Lc-transgenic lines with potent antioxidant activities may provide unique functional products with potential helpful for human health. 
外文关键词：purple-fleshed sweet potato; Lc-transgenic; HPLC-MS; MS; anthocyanins; antioxidant activity
作者：Ge, Jingqiu; Hu, Yijie; Wang, Hongxia; 等.
作者单位：purple-fleshed sweet potato; Lc-transgenic; HPLC-MS; MS; anthocyanins; antioxidant activity
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.463
出版年份：2017
出版刊次：11
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