中文摘要：香蕉枯萎病威胁整个东非的香蕉生产和粮食安全。普通香蕉品种缺乏天然抗性。抗枯萎病转基因香蕉通过插入甜椒的过敏反应促进蛋白（HRAP）或/和植物铁氧还蛋白样蛋白（PFLP）基因研制而成。转基因香蕉对香蕉枯萎病的抗性在田间试验中达到100%。本文基于安全使用蛋白质资源的历史公开资料和对已知的过敏原蛋白建立生物序列比较的方法，评价了抗枯萎病转基因香蕉中HRAP和PFLP蛋白致敏性和毒性的潜在风险。研究发现，转基因香蕉中HRAP和PFLP蛋白不会对人类带来健康风险。
外文摘要：Banana Xanthomonas wilt (BXW) disease threatens banana production and food security throughout East Africa. Natural resistance is lacking among common cultivars. Genetically modified (GM) bananas resistant to BXW disease were developed by inserting the hypersensitive response-assisting protein (Hrap) or/and the plant ferredoxin-like protein (Pflp) gene(s) from sweet pepper (Capsicum annuum). Several of these GM banana events showed 100% resistance to BXW disease under field conditions in Uganda. The current study evaluated the potential allergenicity and toxicity of the expressed proteins HRAP and PFLP based on evaluation of published information on the history of safe use of the natural source of the proteins as well as established bioinformatics sequence comparison methods to known allergens (www.AllergenOnline.org and NCBI Protein) and toxins (NCBI Protein). The results did not identify potential risks of allergy and toxicity to either HRAP or PFLP proteins expressed in the GM bananas that might suggest potential health risks to humans. We recognize that additional tests including stability of these proteins in pepsin assay, nutrient analysis and possibly an acute rodent toxicity assay may be required by national regulatory authorities. (C) 2017 The Authors. Published by Elsevier Ltd.
外文关键词：HRAP; PFLP; Allergen; Toxin; Risk assessment; Genetically modified (GM) bananas
作者：Jin, Yuan; Goodman, Richard E.; Tetteh, Afua O.; 等
作者单位：Int Inst Trop Agr
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.778
出版年份：2017
出版刊次：12
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