中文摘要：油菜是最重要的油料作物之一。为了避免除草剂的威胁，含有bar基因和barstar基因的RF3油菜被研发，它可以作为抗除草剂油菜与恢复系油菜的杂交品种。本文在SD大鼠的90天喂养试验中，分别在饮食中加入2.5%、5%、10%的转基因RF3油菜及其非转基因等基因品种来进行食品安全研究。并对大鼠的一般生长和毒理学参数以及肠道菌群进行了评估。研究发现，大鼠的体重、饲料消耗、相对脏器重量、血液学和血清生化指标有显著差异，但这些统计学差异在不同的大鼠中随机出现，没有剂量相关性，因而认为不具有生物学意义。此外，粪便样品的细菌16S rRNA测序结果表明，含有转基因油菜的饮食不会扰乱肠道菌群的平衡。研究结果表明，基于90天的喂养实验和肠道菌群分析，转基因RF3油菜与非转基因油菜一样是安全且健康的。
外文摘要：Canola is one of the most important plant oilseed crops. To avoid the threat of herbicides, the RF3 line with bar gene and barstar gene was developed, which can act as glufosinate resistance resources and restore fertility in hybrid lines. To assess the food safety of transgenic canola RF3, 2.5%, 5% and 10% GM canola RF3 and its non GM isogenic line Drakkar were formulated into diet to feed Spragure-Dawley (SD) rats for 90 days. The effects on the general growth and toxicological parameters, as well as gut microbiota of rats, were evaluated. Several significant differences on body weight, feed consumption, relative organ weight, hematology and serum biochemistry were observed among rats in the 90-day feeding test. However, these statistical differences were randomly observed among different groups and were not dose-related, which were not considered to be biologically significant. Furthermore, the results of bacterial 16S rRNA sequencing of fecal samples showed that the diets containing GM canola did not disturb the balance of gut microbiota. In conclusion, the canola RF3 is considered as safe and wholesome as the non-GM canola based on this 90-day feeding test and gut microbiota analysis.
外文关键词：Transgenic canola; Safety assessment; 90-Day feeding test; Gut microbiota
作者：Lang, Tianqi; Zou, Shiying; Huang, Kunlun; 等.
作者单位：China Agr Univ
期刊名称：REGULATORY TOXICOLOGY AND PHARMACOLOGY
期刊影响因子：2.221
出版年份：2017
出版刊次：11
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