中文摘要：快速检测饲料样品中的霉菌毒素是农业部门切实执行现行规章制度、保障食品和饲料安全的一个日益相关的挑战。为了实现霉菌毒素的快速检测，已发布了多种生物传感策略，有的甚至仅需几分钟检测时间。然而，这类快速检测生物传感策略依靠基于挥发性有机溶剂的样品制备，通常包括复杂的多步程序并缺乏纯化和/或浓缩作用。本研究报道了一种基于聚乙二醇柠檬酸钠双水相体系的绿色、无毒、经济的新型样品制备法，20分钟内可一步提取并浓缩三种目标霉菌毒素：黄曲霉毒素B1（AFB1）、赭曲霉素A（OTA）及脱氧雪腐镰刀菌烯醇（DON）。其灵敏度符合动物饲料的规定和推荐限度，提供了一种现场高效而安全的半定量霉菌毒素分析法。
外文摘要：The rapid detection of mycotoxins in feed samples is becoming an increasingly relevant challenge for the food production sector, in order to effectively enforce current regulations and assure food and feed safety. To achieve rapid mycotoxin detection, several biosensing strategies have been published, many reaching assay times of the order of a few minutes. However, the vast majority of these rely on sample preparation based on volatile organic solvents, often comprising complex multi-step procedures and devoid of clean-up and/or concentration effects. Here, a novel sample preparation methodology based on a green, non-toxic and inexpensive polyethylene glycol-sodium citrate aqueous two-phase system is reported, providing single-step extraction and concentration of three target mycotoxins within 20 min: aflatoxin B1 (AFB1), ochratoxin A (OTA) and deoxynivalenol (DON). With point-of-need applications in mind, the extraction procedure was optimized and validated using a rapid multi-toxin microfluidic competitive immunoassay. The assay was successfully tested with spiked complex solid matrices including corn, soy, chickpea and sunflower-based feeds and limits of detection of 4.6 ng g(-1) +/- 15.8%, 24.1 ng g(-1) +/- 8.1% and 129.7 ng g(-1) +/- 53.1% (+/- CV) were obtained in corn for AFB1, OTA and DON, respectively. These sensitivities are fit-for-purpose at the required regulatory and recommended limits for animal feed, providing an effective and safe semi-quantitative mycotoxin analysis that can be performed in the field.
外文关键词：ATPS；Mycotoxins；Chemiluminescence；Feeds；Microchannel；Microfluidics
作者：Soares, RRG；Azevedo, AM；Fernandes, P；Chu, V；Conde, JP；Aires-Barros, MR；AF Soares, Ruben R. G.；Azevedo, Ana M.；ernandes, Pedro；Chu, Virginia；Conde, Joao P.
作者单位：Univ Lisbon
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：-
点击下载：一种使用双水相系统提取、浓缩及快速免疫检测饲料中多种霉菌毒素的简便方法