中文摘要：本研究描述了用高效液相色谱结合光化学柱后衍生及荧光检测法测定牛奶中的黄曲霉毒素M1。用该方法检测不同类型的牛奶和奶制品中的AFM1污染，发现仅2个样品受到了黄曲霉毒素M1污染（发生率为10%），而污染的水平低于马来西亚和欧洲的法规限量。
外文摘要：The determination of aflatoxin M-1 in milk using high performance liquid chromatography with photochemical post-column derivatization and fluorescence detection is described. The samples were first extracted and clean-up using the immunoaffinity AFLATEST column originally targeted for aflatoxins B-1, B-2, G(1) and G(2). The separation of aflatoxin M-1 were performed using C18 Hypersil gold (150 mm x 4.6 mm, 511,mu m) column at 40 degrees C under isocratic elution. Fluorescence detector (FLD) was set at 360 nm and 440 nm as excitation and emission, respectively. The use of methanol to replace acetonitrile as the mobile phase resulted in similar to 67% peak area enhancement of AFM(1). The limit of detection (LOD) and quantification (LOQ) of the analytical method after post-column derivatization without evaporation/reconstitution with mobile phase was 0.0085 mu gL(-1) and 0.025 mu gL(-1) respectively. However, LOD and LOQ improved to 0.002 and 0.004 mu gL(-1) respectively with the addition of evaporation/reconstitution step. The method was statistically validated, showing linear response (R-2 > 0.999), good recoveries (85.2-107.0%) and relative standard deviations (RSD) were found to be <= 7%. The proposed method was applied to determine AFM(1) contamination in various types of milk and milk products. Only 2 samples were contaminated with aflatoxin M-1 (10% incidence). However, the contamination level is below the Malaysian and European legislation limits.
外文关键词：Mycotoxin；Aflatoxin M1；HPLC；Post-column derivatization
作者：Shuib, NS；Makahleh, A；Salhimi, SM；Saad, B；AF Shuib, Nor Shifa；Makahleh, Ahmad；Salhimi, Salizawati Muhamad；Saad, Bahruddin
作者单位：Univ Sains Malaysia
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2017
出版刊次：-
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