中文摘要：本研究在ITO电极上逐层沉积制备了纳米金粒子(Au-NPs)功能化的聚（3,4-乙撑二氧噻吩）(PEDOT)生物电极，用以检测黄曲霉毒素B1。研究表明，所制备的免疫电极实际用于30 ng/mL和50 ng/mL的AFB1加标玉米样品时，再现性分别为96.13% 和94.5%。
外文摘要：Gold nanoparticles (Au-NPs) functionalized Poly (3,4-ethylenedioxythiophene) (PEDOT) bioelectrodes were fabricated layer by layer deposition on ITO electrode for detection of aflatoxin B1. The monoclonal anti-aflatoxin antibodies (Anti-AFB1) were immobilized over the surface of PEDOT/Au-NPs/ITO using EDC/NHS coupling. The surface morphology and characteristics of the modified electrodes were investigated by scanning electron microscope and contact angle measurements. The electrochemical analysis of the fabricated immunoelectrode and the immobilization of the antibodies have been evaluated and confirmed by performing Cyclic Voltammetry (CV), Electrochemical Impedance Spectroscopy (EIS) and Fourier Transform Infrared Spectroscopy (FTIR). Decrease in the value of electron transfer resistance (R-et) and increase in the peak current values after incorporation of Au-NPs signifies the enhanced properties of PEDOT embedded Au-NPs. The heterogeneous rate constant (k(s)) and transfer coefficient (alpha) have been determined by using Laviron's method. The fabricated immunosensor exhibits high sensitive amperometric response of 3.72 mu A ng mL(-1) towards AFB(1) concentration in a linear range of 125 ng mL(-1) with detection limit (LOD) of 0.0045 ng mL(-1) and limit of quantification (LOQ) of 0.0156 ng mL(-1). The fabricated immunoelectrode shows a reproducibility of 96.13% and 94.5% towards real maize sample spiked with AFB1 of concentration 30 ng mL(-1)and 50 ng mL(-1), respectively.
外文关键词：Aflatoxin B-1；Poly (3,4-ethylenedioxythiophene)；Cyclic voltammetry；Electrochemical；Impedance spectroscopy
作者：Sharma, A；Kumar, A；Khan, R；AF Sharma, Arati；Kumar, A.；Khan, Raju
作者单位：Tezpur Univ
期刊名称：MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
期刊影响因子：3.42
出版年份：2017
出版刊次：-
点击下载：用金纳米粒修饰的聚（3,4-乙撑二氧噻吩）电化学免疫传感器检测黄曲霉毒素B1