中文摘要：黄酒发酵过程中必然会出现曲霉和青霉。为了黄酒的安全，需要在生产中监测这两种真菌产生的有毒次生代谢物。本研究开发了一种快速而简便的旋流辅助分散液-液微萃取(VADLLME)法，用来提取并浓缩黄酒中主要的曲霉和青霉菌毒素（黄曲霉毒素B1、B2、G1、G2、赭曲霉素A、霉酚酸、杂色曲霉素及震颤真菌毒素）。该方法最大的优点在于操作简单、经济、有机溶剂用量少，已成功应用于黄酒样品的分析，并发现黄酒中霉酚酸发生率高，需要持续监测。
外文摘要：The presence of Aspergillus and Peniciltium species during the fermentation process of rice wine is inevitable. The toxic secondary metabolites produced by the two fungi need to be practically monitored for rice wine safety. In this study, we developed a fast and simple vortex-assisted dispersive liquid-liquid microextraction (VADLLME) for the extraction and preconcentration of major Aspergillus and Penicillium mycotoxins (aflatoxin Bl, aflatoxin B2, aflatoxin Gl, aflatoxin G2, ochratoxin A, mycophenolic acid, sterigmatocystin and verruculogen) in rice wine. The key parameters affecting the extraction efficiency of VADLLME procedure (pH value, type and volume of extraction and dispersive solvents) were optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD) and desirability function. Target compounds were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Under the optimum conditions, good linearity (R-2>0.99) was achieved for all the analytes in the concentration range of 0.5-100 mu g/L. The recoveries for spiked samples at three concentration levels were between 84.5% and 111.2%, with the relative standard deviations (RSD) lower than 20%. The limits of detection (LOD) and quantification (LOO ranged from 0.05 to 0.5 mu g/L and from 0.1 to 1.0 mu g/L, respectively. Simplicity in operation, low cost and low usage of organic solvents were the major advantages of the developed method. The method was successfully applied to the analysis of rice wine samples. It was found that mycophenolic acid had high incidence rate in rice wine, which needed to be continuously monitored.
外文关键词：Vortex-assisted dispersive liquid-liquid；microextraction；Aspergillus and Penicillium mycotoxins；Rice wine；Response surface methodology；LC-MS/MS
作者：Zhao, ZY；Yang, XL；Zhao, XY；Bai, B；Yao, CX；Liu, N；Wang, JH；Zhou, CY；AF Zhao, Zhiyong；Yang, Xianli；Zhao, Xiaoyan；Bai, Bing；Yao, Chunxia；Liu, Na
作者单位：Shanghai Acad Agr Sci
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：-
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