中文摘要：三聚氰胺、先锋霉素和黄曲霉毒素M1是乳制品中三种典型的有害物质，分别代表非法添加剂、抗生素残留及内部的生物毒素。为了保证乳制品安全，需要一种快捷的筛查和检测这些有害物质的方法。本研究提出了一种同时多重检测乳制品中三聚氰胺、先锋霉素和黄曲霉毒素M1的技术。该技术能实现各种有害物质的同时检测，并将在食品安全分析领域具有广阔的应用前景。
外文摘要：Melamine, cephalexin and Aflatoxin M1 are three typical harmful compounds in dairy products, representing illegal additives, residual antibiotics and internal biological toxins respectively. In order to ensure the safety of dairy products, a rapid method is needed to screen and detect these various harmful substances. This work proposed a multiplexed detection technology for testing melamine, cephalexin and Alfatoxin M1 in milk simultaneously. The method involves the visualized microarray technology using 96-well microtitre plate. Multi-target molecules could be detected simultaneously through the competitive immune analysis. Compared to detection methods based on fluorescence or chemoluminescence, the colorimetric technology provides more direct result that can be detected using a commercial scanner. The limit of detection (LoD) was defined at 16.31 ng mL(-1), 8.21 ng mL(-1) and 0.21 ng mL(-1) for melamine, cephalexin and Aflatoxin M1 respectively, which all fit the national standards for maximum residue limits. The accuracy of method was adequate for these three substances. Experiments were also conducted using actual samples; the recovery rates found in the repeatability conditions were 103.1%-106.2% for melamine, 96.6%-98.3% for cephalexin and 105.4%-109.3% for Aflatoxin M1. This technology could achieve the simultaneous detection of various harmful substances and will have broad applications in the food safety analysis field.
外文关键词：Visualized microarray；Silver nanoparticle；Simultaneous detection
作者：Li, ZH；Li, ZM；Jiang, JD；Xu, DK；AF Li, Zhonghui；Li, Zhoumin；Jiang, Jindou；Xu, Danke
作者单位：Nanjing Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.388
出版年份：2017
出版刊次：-
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