中文摘要：本研究记述了一种通用经济的基于适体的荧光猝灭分析，用来检测霉菌毒素玉米赤霉烯酮（ZEN）。用该适体分析成功地检测了（加标）酒精饮料样品、啤酒及葡萄酒中的ZEN，对含量低至1–16 ng/mL的ZEN进行检测，获得了87-96%的回收率。
外文摘要：A versatile and cost-effective aptamer-based fluorescence quenching assay is described for the detection of the mycotoxin zearalenone (ZEN). Exfoliated functional graphene oxide (FGO) of high water-dispersibility is adopted as an effective fluorescence quencher of the fluorescence of FAM. Quenching properties of graphite, graphene oxide (GO) and FGO were investigated, and FGO is found to be the most efficient quencher. FGO therefore was used in an aptamer-based detection format that allows ZEN to be determined in the concentration range of 0.5 to 64 ng/mL with a limit of detection of 0.5 ng/mL. The aptamer assay has good repeatability and reproducibility (n ≥ 4). Selectivity of the aptamer assay against a set of possible interferents is substantiated. This aptasensing assay was successfully applied to the determination of ZEN in (spiked) alcoholic beverage samples, beer and wine, and recovery values in the range of 87 to 96% were obtained for the determination of ZEN at levels as low as 1–16 ng/mL.
外文关键词：Zearalenone；Optical aptasensor；Fluorescein amidite (FAM)；Functional graphene oxide；Aptamer；Fluorescence quenching；Beer；Wine；Alcoholic beverages
作者：Goud, KY；Hayat, A；Satyanarayana, M；Kumar, VS；Catanante, G；Gobi, KV；Marty, JL
作者单位：Univ Perpignan
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：11
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