中文摘要：黄曲霉毒素（AFs）是一类与肝癌相关的致癌物，常见于食物中。食物中黄曲霉毒素的最大限量极低(0.025–20 μg/kg)。快速灵敏地检测食物中浓度如此低的AFs,主要靠有效而选择性地进行AFs的富集处理，这一点极具挑战性，尽管在过去几十年里做了巨大的努力。本研究设计并合成了一种包含广谱AFs单克隆抗体外壳、壳聚糖中间层及磁珠内核的多层纳米结构(表示为AFs-mAb/CTS/Fe3O4)。据认为，新的纳米结构将为检测食物中的AFs提供一种高效、快捷的途径，保护人类免受某些重要肝癌的威胁。
外文摘要：Aflatoxins (AFs) are a class of carcinogens associated with liver cancers which are used to exist in foodstuffs. There are extremely low maximum limits of AFs in foodstuffs (0.025–20 μg/kg). To fast and sensitively detect such low concentration of AFs in foodstuffs is dominated by the efficiency and selectivity of AFs enrichment process, which is extremely challenging although substantial efforts have been made over the past decades. Here we designed and synthesized a multilayer nanoarchitecture composed of a broad-spectrum AFs monoclonal antibody shell, chitosan middle layer and magnetic bead core (denoted AFs-mAb/CTS/Fe3O4). The efficiency of AFs-mAb/CTS/Fe3O4 in extracting AFs has been found to be more than 60 times higher than both conventional immunoaffinity chromatography and solid-phase extraction. Furthermore, the nanocomposite displays excellent selectivity and good reusability apart from outstanding efficiency. Coupling to ultra-performance liquid chromatography–tandem quadrupole mass spectrometry, this new nanoarchitecture enables to probe six AFs as low as 0.003 μg/kg in foodstuffs with free matrix effects, which is nearly 10 times smaller than the regulated maximum tolerated does. It is believed that the new nanoarchitecture would provide an efficient and fast pathway to detect AFs in foodstuffs to protect human being from some critical liver cancers.
作者：Xie, J；Jiang, HY；Shen, JZ；Peng, T；Wang, JY；Yao, K；Sun, SJ；Shao,B；Tang, JW
作者单位：China Agr Univ
期刊名称：ANALYTICAL CHEMISTRY
期刊影响因子：5.886
出版年份：2017
出版刊次：19
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