中文摘要：环（L-丙-L-脯）二肽能抑制产毒真菌产生黄曲霉毒素而不影响真菌的生长。本文研究了环（L-丙-L-脯）二肽抑制黄曲霉产生黄曲霉毒素的作用方式。研究结果表明环（L-丙-L-脯）二肽通过影响黄曲霉的GST功能来抑制黄曲霉毒素的产生，并且AfGST抑制剂可以作为抑制黄曲霉毒素产生的候选剂。
外文摘要：Cyclo(L-Ala-L-Pro) inhibits aflatoxin production in aflatoxigenic fungi without affecting fungal growth. The mode of action of cyclo(L-Ala-L-Pro) in inhibiting aflatoxin production of Aspergillus flavus was investigated. A glutathione S-transferase (GST) of the fungus, designated AfGST, was identified as a binding protein of cyclo(L-Ala-L-Pro) in an experiment performed using cyclo(L-Ala-L-Pro)-immobilized Sepharose beads. Cyclo(L-Ala-L-Pro) specifically bound to recombinant AfGST and inhibited its GST activity. Ethacrynic acid, a known GST inhibitor, inhibited the GST activity of recombinant AfGST and aflatoxin production of the fungus. Ethacrynic acid reduced the expression level of AflR, a key regulatory protein for aflatoxin production, similar to cyclo(L-Ala-L-Pro). These results suggest that cyclo(L-Ala-L-Pro) inhibits aflatoxin production by affecting GST function in A. flavus, and that AfGST inhibitors are possible candidates as selective aflatoxin production inhibitors.
外文关键词：GLUTATHIONE-S-TRANSFERASE；CONTAMINATION；BIOSYNTHESIS；STRATEGIES；NIDULANS；HEALTH；GENES
作者：Iimura, K；Furukawa, T；Yamamoto, T；Negishi, L；Suzuki, M；Sakuda, S
作者单位：Univ Tokyo
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：7
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