中文摘要：本研究制备了一种一次性适配传感装置，用于特异性检测毒性最大的B系伏马毒素—伏马菌素B1（FB1）。该装置成本低、操作简单、灵敏度高、试剂消耗少，如果有可用的特定适配体，则很容易扩展用于其他霉菌毒素的检测。
外文摘要：A disposable aptasensing device for specific detection of fumonisin B1 (FB1), the most toxic of fumonisin B-series, has been fabricated. In this design, the pretreated screen-printed carbon electrode (SPCE) has been firstly covered by a polydimethylsiloxane (PDMS) film with a hole to construct a micro-cell with the conventional three electrodes at the bottom. Subsequently, well-dispersed gold nanoparticles (AuNPs) have been electrodeposited on the working electrodes to serve as excellent matrices for thiolated aptamer immobilization. Since a single nanoparticle could be conjugated with a large number of aptamer probe, the dense decoration of AuNPs on SPCE provided abundant binding sites for aptamer anchoring, finally enhancing the impedance signal effectively. A high affinity between FB1 and its aptamer has been demonstrated by a small association constant (K-a) of 1.19 x 10(9)M(-1) calculated by Langmuir adsorption isotherm. The developed aptasensor showed a good linearity in the range from 10 pg mL(-1) to 50 ng mL(-1) with a low detection limit of 3.4 pg mL(-1) (S/N = 3). The developed aptasensing device is cost-effective, simple in operation, high-sensitive, less reagent consumption, and can be easily expand to other mycotoxins if the specific aptamer is available.
外文关键词：Aptasensing device；Aptamer；Screen-printed carbon electrode；Fumonisin B1；Label-free；Micro-cell
作者：Ren, CC；Li, HM；Lu, XT；Qian, J；Zhu, MY；Chen, W；Liu, Q；Hao, N；Li, HN
作者单位：Jiangsu Univ
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2017
出版刊次：251
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