中文摘要：黄曲霉毒素、脱氧雪腐镰刀菌烯醇、赭曲霉毒素A和玉米赤霉烯酮是最重要的真菌毒素，每一种都可以单独或共同污染谷物。除了促进健康的成分之外，谷粒的外层（麸皮）也是霉菌毒素污染最严重的部分。因此，高纤维小麦制品的消费增加了消费者的健康风险。本研究的目的是开发一种简单可靠的高效液相色谱法同时测定小麦麸皮（WB）中的这些霉菌毒素，并已成功地应用于定量检测希腊不同地区34个WB样品中的真菌毒素。
外文摘要：Aflatoxins, deoxynivalenol, ochratoxin A and zearalenone are the most important mycotoxins that everyone on its own, in groups or simultaneously contaminate cereals. The external layers of cereal grains (bran) apart from health promoting ingredients are also the most contaminated part with reference to mycotoxin's presence. Therefore, consumption of a high fiber wheat-based diet represent an increased risk to consumer's health. The objective of this study was to develop a simple and reliable high performance liquid chromatography method for the simultaneous determination of these mycotoxins in wheat bran (WB). A double extraction was applied with phosphate buffered saline/methanol and for the clean-up a multi-immunoaffinity column was utilized. The detection was carried out with diode-array and fluorescence detectors linked with a post-column photochemical reactor. After optimization of the chromatographic conditions, all mycotoxins were eluted within similar to 26 min. Limits of detection for each mycotoxin (0.12-12.58 mu g/kg) were below the maximum levels provisioned by European Union regulations. Good linearity was observed for the analytes (r(2) >= 0.9980). The recovery of analyzed mycotoxins ranged from 70.2 to 105.8%, with a relative standard deviation <12%. The method was successfully applied to quantify mycotoxins in 34 WB samples obtained after pearling of grains that were collected from different regions of Greece.
作者：Irakli, MN；Skendi, A；Papageorgiou, MD
作者单位：Hellen Agr Org Demeter
期刊名称：JOURNAL OF CHROMATOGRAPHIC SCIENCE
期刊影响因子：1.32
出版年份：2017
出版刊次：7
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