中文摘要：霉菌毒素对动物健康有负面影响。黄曲霉毒素（AFs）是最常见的对大小型动物都有影响的霉菌毒素，是引起与毒素有关的宠物食品召回的常见原因。对黄曲霉毒素中毒的确诊受到检测及量化生物标本宰杀前AFs及其代谢物的有效分析方法的制约。本研究研发并评估了一种测量动物尿液中黄曲霉毒素B1（AFB1）及其代谢物黄曲霉毒素M1（AFM1）和黄曲霉毒素Q1（AFQ1）的尿定量法。该方法准确度高，重复性好，对微小变化具有很强的鲁棒性。但是，由于对低浓度的AFQ1的灵敏性差，于是建议在诊断黄曲霉毒素中毒时，用该方法对动物尿液中的AFB1和AFM1进行定量，而对AFQ1则进行定性检测。
外文摘要：Mycotoxins negatively impact animal health. Aflatoxins (AFs) are the most common mycotoxins affecting both large and small animals and are a common cause of toxin-related pet food recalls. Definitive diagnosis of aflatoxicosis is constrained by a lack of validated ante-mortem analytical methods for detection and quantitation of AFs and their metabolites in biological specimens. Herein, we developed and evaluated a urine-based quantitative method for measurement of aflatoxin B1 (AFB1) and its metabolites aflatoxin M1 (AFM1) and aflatoxin Q1 (AFQ1) in animal urine. (Some of the results have been presented at 59th AAVLD conference, Greensboro, North Carolina, October 13–19th, 2016.) This method uses an immuno-affinity column for clean-up and pre-column derivatization followed by high performance liquid chromatography analysis with fluorescence detection. The method has high selectivity, recovery (>81%) and sensitivity with an instrument limit of detection of 0.20–1.02 pg; instrument limit of quantitation of 0.77–4.46 pg; and a method lower limit of quantitation of 0.30–2.5 ng/mL. The method has high accuracy, repeatability, and is rugged against minor changes. However, because of poor sensitivity of AFQ1 at low concentrations we recommend this method for quantitative determination of AFB1 and AFM1, and for qualitative measurement of AFQ1 in animal urine for diagnosis of aflatoxicosis.
作者：Du, XW；Schrunk, DE；Shao, DH；Imerman, PM；Wang, C；Ensley, SM；Rumbeiha, WK
作者单位：Iowa State Univ
期刊名称：JOURNAL OF ANALYTICAL TOXICOLOGY
期刊影响因子：2.322
出版年份：2017
出版刊次：8
点击下载：通过高效液相色谱结合荧光检测定量测定动物尿液中黄曲霉毒素B1、M1及Q1的方法的实验室研究进展及评估