中文摘要：本研究建立了从豆粕(SBM)中提取过气化物酶(PO)的条件，进行了实验设计方法学研究，以评估搅拌速度时间、PH及提取溶剂体积对酶提取的作用。研究表明纯化的过氧化物酶与基质的亲和性更高，且能减少80%的脱氧雪腐镰刀菌烯醇，比粗提形式高出20%。
外文摘要：This study established the conditions for the extraction of the enzyme peroxidase (PO) from soybean meal (SBM). An experimental design methodology was carried out in order to evaluate the effects of stirring rate time, pH and extracting solvent volume on the enzyme extraction. By using 5 g SBM and 50 mL phosphate buffer 10 mmol L-1 pH 4.7, 60 min stirring rate at 100 rpm, an enzyme with specific activity of 100 U mg(-1) for SBM was obtained. Two techniques of purification were tested and compared for purification of peroxidase from soybean meal: triphasic partition (TPP) and molecular exclusion chromatography. TPP showcased a greater efficiency with 50% recuperation and a purification factor of 13.6. Peroxidase in crude and pure forms was characterized for kinetics, thermodynamics and biochemistry. The parameter of thermal inactivation indicates high stability to exposure time and temperature increase, showing that enzyme activity is not altered by the presence of constituents of the reaction medium. Peroxidase in crude form represented a greater upkeep in activity, keeping 50% activity for 114 days at 0 degrees C. Peroxidase in pure form had greater affinity for substrate and reduced Deoxynivalenol levels by 80%, 20% more than the crude form.
外文关键词：strategies；three-phase partitioning；mycotoxin
作者：Feltrin, ACP；Fontes, MRV；Gracia, HDK；Badiale-Furlong, E； Garda-Buffon, J
作者单位：Univ Fed Rio Grande
期刊名称：QUIMICA NOVA
期刊影响因子：0.617
出版年份：2017
出版刊次：8
点击下载：豆粕过氧化物酶：制备、纯化及在减少脱氧雪腐镰刀菌烯醇含量中的应用