运用盐析辅助液-液萃取结合超高效液相色谱串联质谱检测功能性植物奶中的镰刀菌毒素

Determination of Fusarium toxins in functional vegetable milks applying salting-out-assisted liquid-liquid extraction combined with ultra-high-performance liquid chromatography tandem mass spectrometry

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中文摘要:植物奶因其生理益处而被当作功能性食品。虽然这类产品的消费量大大提高了,但其污染物却很少在立法上受到重视。然而,它们可能因使用污染原料而含有霉菌毒素。本研究提议用超高效液相色谱串联质谱来检测不同的以谷物、豆类和种子为原料的功能性饮料中最相关的镰刀菌毒素(伏马菌素B1和B2、HT-2和T-2毒素、玉米赤霉烯酮、脱氧雪腐镰刀菌烯醇及镰刀菌酮-X)。样品处理包括简单的无需洗涤的盐析辅助液-液萃取。该方法还用来研究市售的植物功能饮料中的这些霉菌毒素,并在燕麦饮料中发现了脱氧雪腐镰刀菌烯醇。
外文摘要:Vegetable milks are considered as functional foods due to their physiological benefits. Although the consumption of these products has significantly increased, they have received little attention in legislation, as regard to contaminants. However, they may contain mycotoxins resulting from the use of contaminated raw materials. In this work, ultra-high performance liquid chromatography tandem mass spectrometry has been proposed for the determination of the most relevant Fusarium toxins (fumonisin B1 and B2, HT-2 and T-2 toxins, zearalenone, deoxynivalenol and fusarenon-X) in different functional beverages based on cereals, legumes and seeds. Sample treatment consisted of a simple salting-out-assisted liquid-liquid extraction with no further clean-up. The method provided limits of quantification between 3.2-57.7 µg L-1, recoveries above 80% and precision with RSD lower than 12%. The method was also applied for studying the occurrence of these mycotoxins in market samples of vegetable functional beverages and deoxynivalenol was found in three oat-based commercial drinks.
外文关键词:Fusarium toxins;vegetable milks;salting-out-assisted liquid-liquid extraction;ultra-high-performance liquid chromatography;mass spectrometry
作者:Hamed, AM;Arroyo-Manzanares, N;Garcia-Campana, AM;Gamiz-Gracia, L
作者单位:Univ Granada
期刊名称:FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子:1.878
出版年份:2017
出版刊次:11
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  1. 编译服务:农产品质量安全
  2. 编译者:虞德容
  3. 编译时间:2018-01-22