中文摘要：本研究建立了一种用液相色谱串联质谱(LC-MS/MS)的简单而快速的同时检测食用油中11种霉菌毒素：黄曲霉毒素B1、B2、G1和G2；伏马菌素B1、B2和B3；赭曲霉素A；玉米赤霉烯酮；脱氧雪腐镰刀菌烯醇和T-2毒素的方法。并期待将该简单、两步制样过程的方法应用于大规模筛选食用油中的霉菌毒素污染。
外文摘要：A simple and rapid method for the simultaneous determination of eleven mycotoxins-aflatoxins B1, B2, G1, and G2; fumonisins B1, B2, and B3; ochratoxin A; zearalenone; deoxynivalenol; and T-2 toxin-in edible oils was established using liquid chromatography tandem mass spectrometry (LC-MS/MS). In this study, QuEChERS, QuEChERS with dispersive liquid-liquid microextraction, and solvent extraction were examined for sample preparation. Among these methods, solvent extraction with a mixture of formic acid/acetonitrile (5/95, v/v) successfully extracted all target mycotoxins. Subsequently, a defatting process using n-hexane was employed to remove the fats present in the edible oil samples. Mass spectrometry was carried out using electrospray ionization in polarity switching mode with multiple reaction monitoring. The developed LC-MS/MS method was validated by assessing the specificity, linearity, recovery, limit of quantification (LOQ), accuracy, and precision with reference to Commission Regulation (EC) 401/2006. Mycotoxin recoveries of 51.6%-82.8% were achieved in addition to LOQs ranging from 0.025 ng/g to 1 ng/g. The edible oils proved to be relatively uncomplicated matrices and the developed method was applied to 9 edible oil samples, including soybean oil, corn oil, and rice bran oil, to evaluate potential mycotoxin contamination. The levels of detection were significantly lower than the international regulatory standards. Therefore, we expect that our developed method, based on simple, two-step sample preparation process, will be suitable for the large-scale screening of mycotoxin contamination in edible oils.
外文关键词：Simultaneous analysis；multiclass mycotoxin；edible oil；LC-MS, MS
作者：Eom, T；Cho, HD；Kim, J；Park, M；An, J；Kim, M；Kim, SH；Han, SB
作者单位：Chung Ang Univ
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2017
出版刊次：11
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