中文摘要：高灵敏度、有选择性地检测真菌毒素对人类健康和食品安全具有重要意义。本文基于链置换扩增技术（SDA）与G-四链体特异性荧光探针扩增技术研发出了一个通用且无标记的真菌毒素检测方法。本文首次通过DNA扩增技术将目标真菌毒素检测转化为高灵敏度的DNA检测。SDA技术用于扩增目标DNA。只有在目标真菌毒素的存在下，才能触发SDA的过程，产生大量的“产品”DNA。“产品”DNA可组装于G-四链体以提高荧光信号。该方法可以区分不同适体的目标霉菌毒素。研究发现，该方法在食品安全领域有很大的应用价值，更有利于人类健康。
外文摘要：Highly sensitive and selective detection of mycotoxins is of great signification for human health as well as food safety. Herein, we present a universal and label-free detection method for mycotoxins based on strand displacement amplification (SDA) and G-quadruplex-specific fluorescence probe amplification. In this design, detection of target mycotoxin is converted to detect DNA that could be detected easily with high sensitivity through DNA amplification technology for the first time. The technique of SDA is used to amplify the interested DNA. Only in the presence of target mycotoxin, could the procedure of SDA be triggered to generate plenty of "product" DNA. The "product" DNA is assembled to G-quadruplex that could enhance the fluorescence of N-methyl mesoporphyrin IX (NMM) as signal carrier. The strategy can distinguish different target mycotoxins due to the employment of aptamer. The limits of detection (LOD) are 17.79 ng/kg for Aflatoxin Ml (AFM1) and 18.98 ng/kg for Ochratoxin A (OTA), respectively. The provided strategy exhibits great beneficial applications in the fields of foodstuffs safety that is more conducive to human health.
外文关键词：Food safety; Label-free; Strand displacement amplification; G-quadruplex; Mycotoxins
作者：Yin, Jinjin; Liu, Yaqing; Wang, Shuo; 等
作者单位：Tianjin Univ Sci & Technol
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：5.401
出版年份：2018
出版刊次：3
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