中文摘要：本文基于超高效液相色谱法与四极杆Orbitrap质谱仪（UHPLC/Q-Orbitrap）提出了一个用于原料奶多霉菌毒素分析的敏感和特异分析方法。本文研究的霉菌毒素包括黄曲霉毒素B1、黄曲霉毒素B2、黄曲霉毒素G1、黄曲霉毒素G2、黄曲霉毒素M1、黄曲霉毒素M2、赭曲霉毒素A、赭曲霉毒素B、玉米赤霉烯酮、玉米赤霉酮、α赤霉烯酮、β赤霉烯酮、α赤霉酮、β赤霉酮等14种。研究发现，与MRM模式的三重四极杆质谱法相比，本文的Q-Orbitrap分析仪提供对前体离子和产物离子的精确定量，因此在不牺牲分析性能的情况下，对分析物的识别提供了更高的置信度。目前，该方法已成功地应用于来自中国5个不同省份的250个原料奶样品的霉菌毒素检测。
外文摘要：This paper presents the development and application of a sensitive and specific method for the analysis of multi-mycotoxins in raw milk using ultrahigh performance liquid chromatography quadrupole orbitrap mass spectrometry (UHPLC/Q-Orbitrap). Mycotoxins (a total of 14) included in this study were aflatoxin B-1, aflatoxin B-2, aflatoxin G(1), aflatoxin G(2), aflatoxin M-1, aflatoxin M-2, ochratoxin A, ochratoxin B, zearalenone, zearalanone, alpha-zeralanol, beta-zeralanol, alpha-zeralenol, and beta-zeralenol. Mycotoxins in milk were extracted and purified with a multi-mycotoxins AOZ immunoaffinity column in a single run, which made the matrix effect be negligible. The deliberate addition of small amount of acetonitrile was found to be beneficial for the extraction of mycotoxins from the complex milk matrix, especially for zearalenone and its derivatives. The final reconstituted extracts were analyzed using ultra high performance liquid chromatography coupled to quadrupole orbitrap mass spectrometry (UHPLC Q-Orbitrap mass spectrometry). The extraction recoveries are in the range of 60-106%, with coefficient of variation <15%. The limits of detection for the analytes are in the range of 0.0003-0.008 mu g/kg. Compared with a triple quadrupole mass method in MRM mode, the Q-orbitrap analyzer provides accurate masses of both the precursor and product ions, thus offers a higher level of confidence in analyte identification without the sacrifice of analytical performance. The method was successfully applied to investigate the occurrence of the 14 mycotoxins in a total of two hundred and fifty raw milk samples collected from 5 different provinces of China.
外文关键词：Ultra high performance liquid chromatography; Quadrupole orbitrap mass spectrometry; UHPLC/Q-Orbitrap; Milk; Mycotoxins; Immunoaffinity column
作者：Mao, Jianfei; Zheng, Nan; Wen, Fang; 等
作者单位：Chinese Acad Agr Sci
期刊名称：FOOD CONTROL
期刊影响因子：3.496
出版年份：2018
出版刊次：2
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