中文摘要：赭曲霉毒素是剧毒污染物，其对人类和动物的健康构成相当大的威胁。赭曲霉毒素主要包括赭曲霉毒素A（OTA）、赭曲霉毒素B（OM）和赭曲霉毒素C（OTC），其总是以混合物的形式存在于食品原料中，且他们在特殊环境下容易相互转化。三种赭曲霉毒素的共现和不同赭曲霉毒素的转换，使单独的赭曲霉毒素特异性检测不能完全满足赭曲霉毒素快速分析的要求。因此，三中赭曲霉毒素的同时检测是必需的。本文首次研发出了基于单克隆抗体（能够同时检测筛选出的OTA、OTB和OTC）的免疫层析法（ICA），该方法可实现对三种赭曲霉毒素的快速同时检测。研究结果表明，本文所研发的ICA可用于三种赭曲霉毒素的现场检测。
外文摘要：Ochratoxins are highly toxic contaminants and considerable threat to the health of humans and animals. Ochratoxins, mainly include ochratoxin A (OTA), ochratoxin B (OM) and ochratoxin C (OTC), always exist in the food raw materials with the form of the mixture and they can convert to each other easily under special environment. The co-occurrence of three ochratoxins and conversion between different types of major ochratoxins make the specific detection for a separate kind of ochratoxin can not fully meet the rapid analysis demand of ochratoxins. Thus, the simultaneous detection for three major ochratoxins is urgent and indispensable. Here, a monoclonal antibody (mAb) which could simultaneously recognize OTA, OTB and OTC was screened out and then based on the mAb, an immunochromatographic assay (ICA) for rapid detection of the three major ochratoxins was first developed. After optimization, the visible limits of detection (vLODs) were 0.05, 0.025, 0.1 ng/mL and the cut-off concentrations were 0.5, 0.25, 0.5 ng/mL for OTA, OTB and OTC, respectively. Finally, the assay was evaluated using ochratoxins spiked maize samples, and results showed that the developed ICA could provide a practical method for the detection of three major ochratoxins on-site. 
外文关键词：Ochratoxins; Co-occurrence; Conversion; Immunochromatographic assay; Simultaneous detection
作者：Zhang, Mengyue; Yan, Lingzhi; Huang, Qiong; 等
作者单位：Northwest A&F Univ
期刊名称：FOOD CONTROL
期刊影响因子：3.496
出版年份：2018
出版刊次：2
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