中文摘要：本文研发了一种基于基于铁磁性纳米粒子（MNP）的玉米赤霉烯酮酶联免疫吸附试验（ELISA）技术。高度分散的磁性纳米颗粒作为载体固定ZEN抗体可通过反应介质中免疫复合物的快速形成、磁场分离、过氧化物酶标记物的异质性检测等完成拟均相模式免疫分析。并将过氧化物酶标记传统比色底物改为高灵敏度化学发光底物以便用于检测特定配合物的形成。作者用该方法对葡萄酒中的玉米赤霉烯酮进行了测定分析。研究结果表明，基于磁浓度和化学发光检测的ELISA分析方法可以作为一种快速分析霉菌毒素和其他有害物质的通用分析工具。
外文摘要：In this study, an enzyme-linked immunosorbent assay (ELISA) of mycotoxin zearalenone (ZEN) based on magnetic iron nanoparticles (MNPs) was developed. Highly dispersed MNPs used as carriers to immobilize anti-ZEN antibodies allowed the immunoassay to be carried out in a pseudo-homogeneous mode through the rapid formation of immune complexes in the reaction media, their separation by a magnetic field, and the heterogeneous detection of the bound peroxidase label. The traditional colorimetric substrate of a peroxidase label was changed to a highly sensitive chemiluminescent one for the detection of specific complexes' formation. The developed analysis consisted of two main stages: first, the interaction of MNP-based immunosorbent with free ZEN in a large-volume sample and its transfer to a smaller volume (preliminary concentration of the analyte) and then binding with ZEN peroxidase complexes. A proposed novel combination of a 50-fold pre-concentration of ZEN with chemiluminescence detection allows for the determination of zearalenone with the detection limit of 4 pg/mL. Therefore, more than an order of magnitude increase of the assay sensitivity was achieved compared with the same assay format with colorimetric detection (0.06 ng/mL) or chemiluminescent MNP-ELISA without a pre-concentration of the analyte (0.1 ng/mL). The analysis was validated for the determination of zearalenone in wine. An assay based on magnetic concentration and chemiluminescent detection can be proposed as a universal analytical tool for the rapid assessment of food contamination by mycotoxins and other hazardous substances.
外文关键词：Zearalenone; ELISA; Magnetic nanoparticles; Pre-concentration; Chemiluminescent peroxidase reaction
作者：Hendrickson, O. D.; Chertovich, J. O.; Zherdev, A. V.; 等
作者单位：Russian Acad Sci
期刊名称：FOOD CONTROL
期刊影响因子：3.496
出版年份：2018
出版刊次：2
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