中文摘要：不同霉菌毒素所需要的质谱方法不同，这是因为它们需适用于代谢物组的靶标和非靶标分析形式。针对靶标代谢组毒素的定量分析，液相色谱-三重四极、Orbitrap（TM）和飞行时间质谱仪都可提供准确的分析结果。在非靶标代谢组学毒素分析中，可将超高效液相色谱与高分辨质谱（HRMS）组合使用，但高分辨质谱所需的质量分辨率会受到质谱仪的扫描速率影响。本文分别用Orbitrap（TM）、飞行时间质谱仪和FT-ICR-MS技术来进行霉菌毒素研究，并对研究结果进行对比。研究发现，目前超高分辨率的FT-ICR-MS是可明确分配基本和公式的唯一方法，但不能与不损失分辨率的超高效液相色谱法相结合。
外文摘要：The huge variety of mycotoxins requires mass spectrometric approaches as they are applied to metabolomics in its targeted and non-targeted analytical forms. For quantitation of mycotoxins in targeted metabolomics, liquid chromatography coupled to triple quadrupole, Orbitrap (TM) or time-of-flight mass spectrometers provide accurate results. For identification in non-targeted metabolomics, combinations of UPLC and high resolution mass spectrometry (HRMS) are desirable, but mass resolution is compromised by the mass analyzer's scan rates, required for UPLC. In this review, the application of Orbitrap (TM), time of-flight and FT-ICR-MS techniques to mycotoxin research is covered and compared to each other. A superior advantage of all HRMS instruments is that they allow a retrospective data treatment thus preserving the information for later reprocessing. Up to date, ultra high resolving FT-ICR-MS is the only method to unequivocally assign elementary sum formulae but cannot be coupled to UPLC without losing resolution power. 
外文关键词：Metabolomics; FT-ICR-MS; Orbitrap; Time of flight; Modified mycotoxins; Mycobolome; High resolution mass spectrometry
作者：Rychlik, Michael; Kanawati, Basem; Schmitt-Kopplin, Philippe
作者单位：Tech Univ Munich
期刊名称：TRAC-TRENDS IN ANALYTICAL CHEMISTRY
期刊影响因子：8.442
出版年份：2017
出版刊次：11
点击下载：食品组学可作为霉菌毒素研究的工具