中文摘要：本研究开发了一种电化学免疫传感器，用来检测玉米样品中的玉米赤霉烯酮（ZEA）。研究表明该电化学免疫传感器有诸多优点，如样品无需前处理、样品量为20μL、试验用时很短以及检测限较低。将该电化学免疫传感器的检测结果与HPLC-荧光检测结果相比较，获得了很好的相关性。该免疫传感器是一种检测玉米样品中ZEA的有效替代工具。
外文摘要：An electrochemical immunosensor is developed to determine zearalenone (ZEA) mycotoxin in maize samples. It is based on the use of a composite, which was prepared from anti-ZEA poly-clonal antibody bonded to gold nanoparticles immobilized on multi-walled carbon nanotubes/polyethyleneimine dispersions. Carbon screen printed-electrodes (CSPE) were used in the electrochemical transduction stage. The immunoassay is based on a direct competitive assay between ZEA in maize samples and ZEA labeled with horseradish peroxidase enzyme (ZEA-HRP). ZEA determination was performed by amperometry, using an applied potential of −0.3 V. The H2O2, which was not consumed by HRP, was reduced at the electrochemical immunosensor surface. Thus, the reduction current was proportional to the amount of ZEA present in samples. All experimental variables involved in the construction of the electrochemical immunosensor were optimized. The linear concentration range is from 1 × 10−4 to 1 × 10−1 ng mL−1. The limit of detection and SC50 were 0.15 pg mL−1 and 2 pg mL−1, respectively. In addition, an acceptable accuracy, with a percentual coefficient of variation (%CV) less than 20%, and recovery percentages close to 105% were found. The electrochemical immunosensor has great advantages such as no pre-treatment of the sample is required, the sample volume is of 20 μL, the experiments require short times and a very low limit of detection is obtained. Results obtained with this electrochemical immunosensor were compared with those determined by HPLC-fluorescence detection, obtaining a very good correlation. The proposed immunosensor is a valuable alternative tool to determine ZEA in maize samples.
外文关键词：Zearale none；Electrochemical immunosensor；Amperometry；Multi-walled carbon；nanotubes/polyethyleneimine dispersions；Maize samples
作者：Riberi, WI；Tarditto, LV；Zon, MA；Arevalo, FJ；Fernandez, H
作者单位：Univ Nacl Rio Cuarto
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2018
出版刊次：1
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