中文摘要：黄曲霉毒素B1（AFB1）是食品，特别是谷物、油菜籽、香料和坚果中最常见的霉菌毒素之一。过去10年里，适配子蓬勃发展，并在各种检测中代替了抗体，以一种新型生物传感元件出现。本文报道了一种使用AFB1-特异性适配子检测AFB1的更快、更灵敏、高通量的方法。研究表明，与标准的HPLC分析法相比，该传感器的检测灵敏度更高。该检测技术可能开发成检测AFB1的生物传感器平台。
外文摘要：Aflatoxin B1 (AFB1) is one of the most commonly found mycotoxins in food commodities, particularly cereals, oilseeds, spices and tree nuts. In the past decade, aptamers have come into limelight and emerged as a new biosensing element replacing antibodies in various detection formats. Herein we report a faster, more sensitive, high throughput method for the detection of AFB1 using AFB1-specific aptamers. The assay format was based on a competitive reaction of the fluorescent tagged aptamer specific to AFB1 with the aflatoxin conjugate. Under optimal conditions, a linear range of detection (50 ng to 50 pg) was achieved with a limit of detection (LOD) of 10 pg/mL in the buffer system. Results of inter- and intra-assay revealed that the assay was repeatable with standard deviation in acceptable range. The assay was also validated in food samples such as dried red chilies, groundnut and whole pepper with recovery in the range of 92 to 102% at 10 ng/mL and 100 pg/mL levels. The aptasensor assay was also compared with standard analytical method of HPLC and was found to be more sensitive. This detection technique has the potential to be developed into a biosensor platform for AFB1 detection.
外文关键词：Aflatoxin B-1；aptasensor；competitive binding；fluorescence；food samples
作者：Mukherjee, M；Bhatt, P；Manonmani, HK
作者单位：CSIR Cent Food Technol Res Inst CFTRI
期刊名称：JOURNAL OF MOLECULAR RECOGNITION
期刊影响因子：2.091
出版年份：2017
出版刊次：12
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