中文摘要：本文描述了一种新的基于荧光能量共振转移(FRET)的纳米探针，通过开-关信令过程选择性检测黄曲霉毒素B1（AFB1）的方法。该方法已成功应用于（加标）小麦粉中AFB1的检测，并发现其结果与用酶联免疫分析得到的结果一致。
外文摘要：The authors describe a new method for the selective detection of aflatoxin B1 (AFB1) by an off-on signaling procedure in a fluorescence resonance energy transfer (FRET)-based nanobioprobe. An amino-modified aptamer against AFB1 was conjugated to fluorescent polymer dots, containing poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(1,4-benzo-thiadiazole)] as the fluorophore. Complementary DNA (cDNA) was conjugated to silver nanoparticles (cDNA-AgNPs) which act as FRET acceptors. Mixed in solution, in the absence of AFB1, the aptamer and its cDNA hybridize to form (aptamer-cDNA). This brings the polymer dots into close proximity of the AgNPs and result in FRET from the donor to the acceptor due to spectral overlap between the emission of the polymer dots and the absorption of the AgNPs. The fluorescence of the polymer dots probe is thereby switched off. However, in the presence of AFB1, the aptamer with high affinity for AFB1 will be released from the cDNA-AgNP aggregate, which results in recovery of fluorescence (“switch on” state). The yellow fluorescence of the polymer dots, best measured at 538 nm, increases linearly in the 5 pg·mL−1 to 1.0 ng·mL−1 AFB1 concentration range, with a 0.3 pg·mL-1 detection limit. The assay was successfully applied to the detection of AFB1 in (spiked) wheat flour, and the results were found to be in satisfactory agreement with those obtained by an enzyme-linked immunosorbent assay.
外文关键词：Aflatoxin B1；Nano-bio-probe；Polymer dots；Silver nanoparticles；Aptamer；DNA；Fluorescence resonance energy transfer；Nanostructures
作者：Nasirian, V；Chabok, A；Barati, A；Rafienia, M；Arabi, MS；Shamsipur, M
作者单位：Razi Univ
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2017
出版刊次：12
点击下载：用从荧光聚合物点标记的适体到互补DNA标记的银纳米粒子的FRET超灵敏检测黄曲霉毒素B1