中文摘要：本研究基于5-羧基荧光素(FAM)与钯纳米颗粒间的荧光共振能量转移(FRET)，构建了一种高灵敏检测黄曲霉毒素M1（AFM1）的适体传感器。研究表明，该高灵敏FRET适体传感器配置简单，在食品污染物检测中前景广阔。
外文摘要：A highly sensitive aptasensor for aflatoxin M₁ (AFM₁) detection was constructed based on fluorescence resonance energy transfer (FRET) between 5-carboxyfluorescein (FAM) and palladium nanoparticles (PdNPs). PdNPs (33 nm) were synthesized through a seed-mediated growth method and exhibited broad and strong absorption in the whole ultraviolet-visible (UV-Vis) range. The strong coordination interaction between nitrogen functional groups of the AFM₁ aptamer and PdNPs brought FAM and PdNPs in close proximity, which resulted in the fluorescence quenching of FAM to a maximum extent of 95%. The non-specific fluorescence quenching caused by PdNPs towards fluorescein was negligible. After the introduction of AFM₁ into the FAM-AFM₁ aptamer-PdNPs FRET system, the AFM₁ aptamer preferentially combined with AFM₁ accompanied by conformational change, which greatly weakened the coordination interaction between the AFM₁ aptamer and PdNPs. Thus, fluorescence recovery of FAM was observed and a linear relationship between the fluorescence recovery and the concentration of AFM₁ was obtained in the range of 5-150 pg/mL in aqueous buffer with the detection limit of 1.5 pg/mL. AFM₁ detection was also realized in milk samples with a linear detection range from 6 pg/mL to 150 pg/mL. The highly sensitive FRET aptasensor with simple configuration shows promising prospect in detecting a variety of food contaminants.
外文关键词：aflatoxin M-1；palladium nanoparticles；aptasensor；fluorescence resonance energy transfer
作者：Li, H；Yang, DB；Li, PW；Zhang, Q；Zhang, W；Ding, XX；Mao, J；Wu, J
作者单位：Chinese Acad Agr Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：10
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