中文摘要：大多数热带水果采摘后容易感染真菌而腐坏。由于病原菌能产生有害物质如霉菌毒素，因而迫切需要开发新的快速有效的方法来评估这些有害物质。本研究报道了一种发光细菌，青海弧菌Q67，它可以用来快速评估产霉菌毒素病原菌产生的霉菌毒素及其培养物的毒性。研究表明青海弧菌Q67是一种有前途的快速评估真菌毒素毒性的方法。
 
外文摘要：Most tropical fruits after harvest are very perishable because of fungal infection. Since some pathogenic fungi can produce hazardous compounds such as mycotoxins, novel rapid and effective methods to assess those hazardous compounds are urgently needed. Herein we report that Vibrio qinghaiensis sp. Q67, a luminescent bacterium, can be used to rapidly assess the toxicities of mycotoxins and cultures from mycotoxin-producing pathogens. A good correlation (R² > 0.98) between concentrations of the mycotoxins (fumonisin B1, deoxynivalenol, zearalenone, ochratoxin A, patulin, and citrinin) and the luminous intensity of V. qinghaiensis sp. Q67 was obtained. Furthermore, significant correlations (R² > 0.96) between the amount of mycotoxin and the luminous intensity from the cultures of 10 major mycotoxin-producing pathogens were also observed. In addition, Fusarium proliferatum (half-maximal inhibitory concentration (IC50) = 17.49%) exhibited greater luminescence suppression than Fusarium semitectum (IC50 = 92.56%) or Fusarium oxysporum (IC50 = 28.61%), which was in agreement with the existing higher levels of fumonisin B1, fumonisin B2, and deoxynivalenol, which were measured by high-performance liquid chromatography-tandem mass spectrometry. These results suggest that V. qinghaiensis sp. Q67 is a promising alternative for the rapid evaluation of the toxicity of fungal mycotoxins.
外文关键词：toxicity；mycotoxin；bioluminescence；Vibrio qinghaiensis sp；Q67
作者：Jian, QJ；Gong, L；Li, TT；Wang, Y；Wu, Y；Chen, F；Qu, HX；Duan, XW；Jiang, YM
作者单位：Chinese Acad Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：10
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