中文摘要：本研究优化了一种荧光偏振免疫分析法(FPIA)并用它检测了黑麦及黑麦薄脆饼干中的赭曲霉毒素A（OTA）。结果表明优化后的FPIA适用于对黑麦及黑麦薄脆饼干中低于欧盟审管限值水平的OTA进行高通量筛查及可靠的定量测定。
外文摘要：A rapid fluorescence polarization immunoassay (FPIA) was optimized and validated for the determination of ochratoxin A (OTA) in rye and rye crispbread. Samples were extracted with a mixture of acetonitrile/water (60:40, v/v) and purified by SPE-aminopropyl column clean-up before performing the FPIA. Overall mean recoveries were 86 and 95% for spiked rye and rye crispbread with relative standard deviations lower than 6%. Limits of detection (LOD) of the optimized FPIA was 0.6 μg/kg for rye and rye crispbread, respectively. Good correlations (r > 0.977) were observed between OTA contents in contaminated samples obtained by FPIA and high-performance liquid chromatography (HPLC) with immunoaffinity cleanup used as reference method. Furthermore, single laboratory validation and small-scale collaborative trials were carried out for the determination of OTA in rye according to Regulation 519/2014/EU laying down procedures for the validation of screening methods. The precision profile of the method, cut-off level and rate of false suspect results confirm the satisfactory analytical performances of assay as a screening method. These findings show that the optimized FPIA is suitable for high-throughput screening, and permits reliable quantitative determination of OTA in rye and rye crispbread at levels that fall below the EU regulatory limits.
外文关键词：fluorescence polarization immunoassay；ochratoxin A；rye；rye-based products；validation study
作者：Lippolis, V；Porricelli, ACR；Cortese, M；Suman, M；Zanardi, S；Pascale, M
作者单位：CNR
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：10
点击下载：用荧光偏振免疫分析测定黑麦及黑麦制品中的赭曲霉毒素A