中文摘要：本研究采用气相色谱-质谱联用代谢组学方法，旨在评估饲喂了含或不含玉米赤霉烯酮（ZEN）和杂色曲霉素（STC）污染日粮的牛的代谢谱。从饲料有毒的牛得到的结果来看，为期两周的治疗周期不足以改善尿代谢物水平，说明霉菌毒素慢性污染可能会对牛的全身代谢产生长期的不良影响。
外文摘要：The objective of this study was to evaluate the metabolic profile of cattle fed with or without zearalenone (ZEN) and sterigmatocystin (STC)-contaminated diets using a gas chromatography-mass spectrometry metabolomics approach. Urinary samples were collected from individual animals (n = 6 per herd) from fattening female Japanese Black (JB) cattle herds (23 months old, 550-600 kg). Herd 1 had persistently high urinary ZEN and STC concentrations due to the presence of contaminated rice straw. Herd 2, the second female JB fattening herd (23 months old, 550-600 kg), received the same dietary feed as Herd 1, with non-contaminated rice straw. Urine samples were collected from Herd 1, two weeks after the contaminated rice straw was replaced with uncontaminated rice straw (Herd 1N). Identified metabolites were subjected to principal component analysis (PCA) and ANOVA. The PCA revealed that the effects on cattle metabolites depended on ZEN and STC concentrations. The contamination of cattle feed with multiple mycotoxins may alter systemic metabolic processes, including metabolites associated with ATP generation, amino acids, glycine-conjugates, organic acids, and purine bases. The results obtained from Herd 1N indicate that a two-week remedy period was not sufficient to improve the levels of urinary metabolites, suggesting that chronic contamination with mycotoxins may have long-term harmful effects on the systemic metabolism of cattle.
外文关键词：cattle；GC；MS；metabolomics；sterigmatocystin；urine；zearalenone
作者：Toda, K；Kokushi, E；Uno, S；Shiiba, A；Hasunuma, H；Fushimi, Y；Wijayagunawardane, MPB；Zhang, C；Yamato, O；Taniguchi, M; Fink-Gremmels, J；Takagi, M
作者单位：Yamaguchi Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2017
出版刊次：10
点击下载：用气相色谱-质谱法分析受玉米赤霉烯酮和杂色曲霉毒素自然污染的日本和牛的代谢谱