中文摘要：本研究开发并验证了一种运用超高效液相色谱-串联质谱(UHPLC-MS/MS)，测定供婴儿食用的麦片粥中的黄曲霉毒素M2、M1、G2、G1、B2、B1、脱氧雪腐镰刀菌烯醇、赭曲霉毒素A、伏马菌素B1和B2、水解伏马菌素B1和B2、玉米赤霉烯酮及杂色曲霉素的分析方法。研究发现，所分析的样品中，至少有一种霉菌毒素超出了巴西立法规定的最大允许限度的有21个（25%）。
外文摘要：An analytical method applying ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for the determination of aflatoxins M2, M1, G2, G1, B2, B1, deoxynivalenol, ochratoxin A, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, zearalenone and sterigmatocystin in cereal-based porridge destined for infant consumption was developed and validated. The mycotoxins were extracted using an adequate solvent ratio (n-hexane/3% formic acid solution/acetonitrile) under rapid shaking and sonication, without any further cleanup steps. Recoveries ranged from 63.5 to 113.2% and were considered satisfactory, with relative standard deviations lower than 20%. The limits of quantification ranged from 0.14 to 6.73 μg kg−1. The validated method was then applied to the determination of mycotoxins in 84 samples of cereal-based porridge destined for infant consumption obtained in the metropolitan region of Rio de Janeiro, RJ. Aflatoxins B1 and G1, fumonisins B1 and B2, hydrolyzed fumonisins B1 and B2, deoxynivalenol, and zearalenone were found, respectively, in 7.1, 2.4, 47.6, 3.6, 65.5, and 60.7% of the analyzed samples. The maximum permissible limits set by the Brazilian legislation were exceeded for at least one mycotoxin in 21 (25%) of the analyzed samples.
外文关键词：Mycotoxins；Infant food；Cereal-based porridge；UHPLC-MS/MS
作者：Sartori, AV；de Moraes, MHP；Santos, R；Souza, YP；da Nobrega, AW
作者单位：Oswaldo Cruz Fdn INCQS FIOCRUZ
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：12
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