中文摘要：本研究开发了一种运用简单、快捷而经济的分散磁性Fe3O4纳米颗粒固相萃取的预浓缩技术，用来提取并测定坚果和谷物样品中的黄曲霉毒素B1、B2、G1和G2。研究表明，该方法简单经济，可替代常规检测法，并适用于食品样品中污染物的检测。
外文摘要：The application of a simple, fast, and cheap sample pre-concentration technique based on the dispersive magnetic Fe3O4 nanoparticle solid-phase extraction has been developed for extraction and determination of aflatoxins B1, B2, G1, and G2 in nut and cereals. Aflatoxins were extracted from samples with a mixture of methanol–water 8:2 (v/v) and then by magnetic Fe3O4 nanoparticles and a 500 μL of chloroform; pre-concentration and cleanup of the analytes simultaneously were done. HPLC with fluorescence detector and electrochemical post-column derivatization has been used for aflatoxin determination. The main parameters affecting the extraction efficiency, such as type and volume of extraction solvent, the volume of water, and sample volume, were investigated and optimized to achieve the best extraction efficiency. The LODs and LOQs of the proposed method for the AFs were determined, which LODs were 0.35 and 0.06 μg/kg, and LOQs were 1 and 0.2 μg/kg, for aflatoxin groups B and G, respectively. Acceptable recoveries were achieved (76.0–112.7%), and the low limits of detection (LOD <0.35 and 0.06 μg/kg), with proper precision (RSD <15%), were obtained. Therefore, the validated method represents simple and low-cost substitute to the conventional method and is suitable for screening of contamination in food samples.
外文关键词：Aflatoxins；HPLC；Nanomagnetic particles；Pistachio；Rice
作者：Karami-Osboo, R；Mirabolfathi, M
作者单位：Iranian Res Inst Plant Protect
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2017
出版刊次：12
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