中文摘要：本研究用开发了一种新型的多巴胺脂质体（DLL）信号放大策略，用Mn2+掺杂Zn3(OH)2V2O7·2H2O纳米带对食品中小分子黄曲霉毒素B1(AFB1)进行竞争型非酶光电化学(PEC)免疫分析。研究表明该系统为小分子检测提供了一种理想的基于Mn2+掺杂Zn3(OH)2V2O7·2H2O纳米带和高脂质体的PEC免疫传感平台。
外文摘要：A novel signal-amplified strategy based on dopamine-loaded liposome (DLL) was developed for competitive-type nonenzymatic photoelectrochemical (PEC) immunoassay of small- molecular aflatoxin B1 (AFB1) in foodstuff, using Mn2+-doped Zn-3(OH)(2)V2O7 center dot 2H(2)O nanobelts. The signal was amplified by high-loaded capacity of liposome and the highly efficient dopamine molecule to enhance photocurrent of Mn2+-doped Zn-3(OH)(2)V2O7 center dot 2H(2)O nanobelts. The loaded dopamine in the liposome was used as an electron donor to scavenge the hole and inhibit the charge recombination. To design such an immunoassay system, a AFB1-bovine serum albumin (AFB1-BSA) conjugate was covalently bound with the multifunctional liposome via the cross-linkage glutaraldehyde, whereas monoclonal anti-AFB1 antibody was labeled onto a magnetic bead by typical carbodiimide coupling. Upon addition of target AFB1, a competitive immunoreaction was carried out between the analyte and the AFB1-BSA-DLL for the conjugated antibody on the magnetic bead. Followed by magnetic separation, the carried DLL on the magnetic bead was lysed by using Triton X-100 to release the encapsulated dopamine. The as-produced dopamine (as an elector donor) increased the photocurrent of the Mn2+-doped Zn-3(OH)(2)V2O7 center dot 2H(2)O nanobelts. The photocurrent depended on the as-released amount of the dopamine. The change in the photocurrent enhanced with the increasing AFB1 concentration. Under the optimal conditions, Mn2+-doped Zn-3(OH)(2)V2O7 center dot 2H(2)O nanobelts exhibited good photoelectrochemical responses for the detection of AFB1 and allowed the detection of AFB1 at a concentration as low as 0.3 pg mL(-1) within a linear range from 0.5 pg mL(-1) to 10 ng mL(-1). Importantly, this system provided an ideal PEC immune sensing platform based on Mn2+-doped Zn-3(OH)(2)V2O7 center dot 2H(2)O nanobelts and the high-loaded liposome for the detection of small molecules.
作者：Lin, YX；Zhou, Q；Tang, DP
作者单位：Fuzhou Univ
期刊名称：:ANALYTICAL CHEMISTRY
期刊影响因子：5.886
出版年份：2017
出版刊次：21
点击下载：多巴胺脂质体用于AFB1的原位放大光电化学免疫检测，提高Mn2+掺杂 Zn3(OH)2V2O7纳米带的光电流