中文摘要：黄曲霉毒素和玉米赤霉烯酮（ZEN）是玉米及其制品中非常常见的霉菌毒素。本研究旨在报道一种用两种独特型纳米抗体研发的时间分辨荧光免疫层析(TRFICA)，快速、定量、同时检测玉米及其制品中的黄曲霉毒素B1（AFB1）和ZEN。研究表明，Aldnb TRFICA法检测样品具有良好的回收率(72.6%-106.6%)，用它检测玉米样品中的双重霉菌毒素，结果令人满意。据悉，用基于AIdnbs的时间分辨层析法检测双重霉菌毒素，本报道尚属首例。
外文摘要：Aflatoxins and zearalenone (ZEN) are highly common mycotoxins in maize and maize-based products. This study aimed to report a time-resolved fluorescence immunochromatographic assay (TRFICA) developed using two idiotypic nanobodies for rapid, quantitative, and simultaneous detection of aflatoxin B-1 (AFB(1)) and ZEN in maize and its products. A novel Eu/Tb(III) nanosphere with enhanced fluorescence was prepared as a label and conjugated to anti-idiotypic nanobody (AIdnb) and monoclonal antibody (mAb). On the basis of nanosphere-antibody conjugation, two patterns of competitive time-resolved strip methods (AIdnb-TRFICA and mAb-TRFICA) were established and compared. The half inhibition concentration of AIdnb-TRFICA was 0.46 and 0.86 ng rnL(-1) for AFB(1) and ZEN, which was 18.3- and 20.3-fold more sensitive than that of mAb-TRFICA for AFB(1) and ZEN, respectively. Under optimal conditions, AIdnb-TRFICA for dual mycotoxin was established and provided a quantitative relationship ranging from 0.13 to 4.54 ng.mL(-1) for AFB1 and 0.20 to 2.77 ng.mL(-1) for ZEN, with a detection limit of 0.05 and 0.07 ng.mL(-1) in the buffer solution, respectively. Aldnb TRFICA showed good recoveries (72.6%-106.6%) in samples and was applied to detect dual mycotoxin in maize samples with satisfying results. To the best of our knowledge, it is the first report about a time-resolved strip method based on AIdnbs for dual mycotoxin.
作者：Tang, XQ；Li, PW；Zhang, Q；Zhang, ZW；Zhang, W；Jiang, J
作者单位：Chinese Acad Agr Sci
期刊名称：ANALYTICAL CHEMISTRY
期刊影响因子：5.886
出版年份：2017
出版刊次：21
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