中文摘要:因产生有害霉菌毒素,特别是脱氧雪腐镰刀菌烯醇(DON),小麦赤霉病(FHB)会造成严重的谷物损失和谷物污染。关于各小麦品种中镰刀菌抗性和DON毒素积累的研究非常广泛;然而,DON-3-O-葡萄糖苷(D3G)的相关研究尚不充分。本研究利用内部验证的分析方法测量了CIMMYT小麦优良种质中DON和D3G累积的水平。并分析了共生的雪腐镰刀菌醇(NIV)和麦角菌素(ERG)情况。
外文摘要:Fusarium head blight (FHB) causes significant grain loss and contamination of grains with harmful mycotoxins, especially deoxynivalenol (DON). Fusarium resistance and DON accumulation have been extensively investigated in various cultivars; however, the level of DON-3-O-glucoside (D3G) has not been as carefully studied. In this study, we measured accumulated DON and D3G levels in CIMMYT wheat elite germplasm using an analytical method validated in-house. Co-occurring nivalenol (NIV) and ergostrerol (ERG) were also analyzed. LC-MS/MS and LC-UV analyses were applied to the 50 CIMMYT elite wheat lines. D3G showed rather high correlation with DON (r = 0.82), while FHB symptoms showed slight correlation with DON and D3G (r = 0.36 and 0.32, respectively). D3G/DON ratio varied widely from 8.1 to 37.7%, and the ratio was not related with FHB resistance in this dataset.
外文关键词:Fusarium head blight (FHB);deoxynivalenol (DON);deoxynivalenol-3-O-glucoside (D3G);high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS)
作者:Nakagawa, Hiroyuki;He, Xinyao;Matsuo, Yosuke
作者单位:National Agriculture & Food Research Organization
期刊名称:TOXINS
期刊影响因子:3.571
出版年份:2017
出版刊次:8
点击下载:CIMMYT小麦种质中脱氧雪腐镰刀菌烯醇的隐性代谢物和镰刀菌抗性分析