中文摘要：本文提出的协同研究方法旨在评估用稳定同位素稀释法（SIDA）和LC-MS/MS方法同步测定食品中黄曲霉毒素B1、B2、G1和G2，脱氧雪腐镰刀菌烯醇，伏马菌素B1、B2和B3，赭曲霉毒素A，HT-2毒素，T-2毒素以及玉米烯酮等的效果。
外文摘要：A collaborative study was conducted to evaluate stable isotope dilution assay (SIDA) and LC-MS/MS for the simultaneous determination of aflatoxins B-1, B-2, G(1), and G(2); deoxynivalenol; fumonisins B-1, B-2, and B-3; ochratoxin A; HT-2 toxin; T-2 toxin; and zearalenone in foods. Samples were fortified with 12 C-13 uniformly labeled mycotoxins (C-13-IS) corresponding to the native mycotoxins and extracted with acetonitrile/water (50:50 v/v), followed by centrifugation, filtration, and LC-MS/MS analysis. In addition to certified reference materials, the six participating laboratories analyzed corn, peanut butter, and wheat flour fortified with the 12 mycotoxins at concentrations ranging from 1.0 to 1000 ng/g. Using their available LC-MS/MS platform, each laboratory developed in-house instrumental conditions for analysis. The majority of recoveries ranged from 80 to 120% with relative standard derivations (RSDs) <20%. Greater than 90% of the average recoveries of the participating laboratories were in the range of 90-110%, with repeatability RSDr (within laboratory) < 10% and reproducibility RSDR (among laboratory) < 15%. All Z scores of the results of certified reference materials were between -2 and 2. Using C-13-IS eliminated the need for matrix-matched calibration standards for quantitation, simplified sample preparation, and achieved simultaneous identification and quantitation of multiple mycotoxins in a simple LC-MS/MS procedure.
外文关键词：mycotoxin；LC-MS/MS；stable isotope dilution assay (SIDA)
作者：Zhang, Kai；Schaab, Matthew R.；Southwood, Gavin
作者单位：US Food & Drug Administration
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：33
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