中文摘要：本研究开发并验证了一种易操作、基于膜的筛选多重霉菌毒素的flow-through方法，该方法可用于各种谷物饲料成分和复合饲料，如小麦、大麦、大豆、麦麸、水稻、米糠、玉米、菜籽粉和葵子饼粉，以及各种类型的全价配合饲料（如小鸭饲料、猪饲料、肉鸡饲料、猪仔饲料）中四种霉菌毒素（玉米烯酮、脱氧雪腐镰刀菌烯醇、黄曲霉毒素B1和赭曲霉毒素A）进行多重筛选。
外文摘要：An easy-to-operate membrane-based flow-through test for multiplex screening of four mycotoxins (zearalenone, deoxynivalenol, aflatoxin B-1, and ochratoxin A) in a variety of cereal-based feed ingredients and compound feeds, such as wheat, barley, soybean, wheat bran, rice, rice bran, maize, rapeseed meal, and sunflower meal, and various types of complete feed (duckling feed, swine feed, broiler feed, piglet feed) was developed and validated. First, the antibodies were evaluated by enzyme-linked immunosorbent assay and then employed in the membrane rapid test. The cutoff levels for zearalenone, deoxynivalenol, aflatoxin B-1, and ochratoxin A were 50, 200, 1, and 10 mu g/kg, respectively, based on European regulations and consumers' requirements. As sample pretreatment, consecutive steps of extraction, dilution, solid-phase extraction by addition of C18 sorbent, and final filtration of supernatant were followed. Both the sample preparation and the analysis procedure were simple, cost-effective, and easy to perform on-site in a nonlaboratory environment. The impact of sample processing on the result of the experiment was investigated supported by experimental design. The validation procedure was performed on the basis of Commission Regulation 2006/401/EC. The numbers of false-positive and false-negative outcomes were < 5%, going along with the Commission Decision 2002/657/EC. Liquid chromatography-tandem mass spectrometry was performed as a confirmatory technique.
外文关键词：multiassay；membrane test；flow-through；rapid test；immunoassay；multiplex screening；mycotoxins
作者：Beloglazova, Natalia V.；Graniczkowska, Kinga；Ediage, Emmanuel Njumbe
作者单位：Ghent University
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：33
点击下载：一种灵敏的Flow-through免疫分析法，可以快速多重检测饲料和饲料原料中谷物携带的霉菌毒素