中文摘要：本研究开发了以发光量子点（QDs）为标记的多重侧流免疫分析法（LFIA），用于测定大麦中的脱氧雪腐镰刀菌烯醇、玉米烯酮和T2/HT2毒素。LFIA法速度快（15分钟）、假阴性率低（<5%），且结果易解释，不需要任何复杂的设备。
外文摘要：A multiplex lateral flow immunoassay (LFIA) for the determination of the mycotoxins deoxynivalenol, zearalenone, and T2/HT2-toxin in barley was developed with luminescent quantum dots (QDs) as label. The synthesized QDs were hydrophilized by two strategies, that is, coating with an amphiphilic polymer or silica. The water-soluble QDs were compared with regard to their bioconjugation with monoclonal antibody (mAb) and were tested on a LFIA. Silica-coated QDs that contained epoxy groups were most promising. Therefore, green, orange, and red epoxy-functionalized silica-coated QDs were conjugated with anti-ZEN, anti-DON, and anti-T2 mAb, respectively. The LFIA was developed in accordance with the European Commission legal limits with cutoff limits of 1000, 80, and 80 mu g/kg for deoxynivalenol, zearalenone, and T2/HT2-toxin, respectively. The LFIA gave a fast result (15 min) with a low false-negative rate (<5%), and the results were easy to interpret without any sophisticated equipment.
外文关键词：quantum dots；bioconjugation；multiplex lateral flow immunoassay；mycotoxin
作者：Foubert, Astrid；Beloglazova, Natalia V.；Gordienko, Anna
作者单位：Ghent University
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：2.857
出版年份：2017
出版刊次：33
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