中文摘要：本研究采用简易的样品制备程序，研发了一种间接竞争酶联免疫吸附试验(ic-ELISA)的广谱单克隆抗体(mAb)，用来快速筛查各种样品中的玉米赤霉烯酮及其主要类似物。研究表明用ic-ELISA法和HPLC-MS/MS法检测猪饲料的结果具有良好的相关性(R2=0.995)，是对该ic-ELISA法可靠性的有力支持。
外文摘要：A broad-spectrum monoclonal antibody (mAb)-based indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed for rapidly screening zearalenone (ZEN) and its primary analogues in various samples using an easy sample preparation procedure. Primarily, a group-specific mAb, 6C2, was produced, which had IC50 values for ZEN, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol and zearalanone of 114.0, 127.4, 290.4, 114.9, 205.6 and 257.1 ng L-1, respectively. The limit of detection and limit of quantitation of this method for ZEN and its five primary analogues in various matrix samples ranged from 114.2 to 812.3 ng L-1 and 237.1 to 1653.9 ng L-1, respectively. The recoveries of the above samples spiked with ZEN and its five primary analogues were in the range of 62.9-113.6%. The CVs were less than 13.2%. A good correlation (R2 = 0.995) between the ic-ELISA results and the HPLC-MS/MS results for swine feeds supported the reliability of the developed ic-ELISA.
外文关键词：Zearalenone；Monoclonal antibody；Indirect competitive enzyme-linked immunosorbent assay；Feed；Animal edible tissue
作者：Dong, GL；Pan, YH；Wang, YL；Ahmed, S；Liu, ZL；Peng, DP；Yuan, ZH
作者单位：Huazhong Agr Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2018
出版刊次：5
点击下载：玉米赤霉烯酮及其主要类似物广谱抗体的制备及其在间接竞争酶联免疫吸附试验中的应用