中文摘要：本研究提出了一种用旋转圆盘电极改进的单链DNA(ss-HSDNA/rGOae)标记的还原氧化石墨气凝胶阻抗生物传感器，用来测定黄曲霉毒素B1（AFB1）的水平。研究发现，提高转速降低了电荷转移的电阻，从而可以测定更低水平的AFB1。
外文摘要：Here, an impedimetric biosensor for determination and quantifica-tion of an aflatoxin B1 (AFB1) level using a reduced graphene oxide aerogel labeled with a single strand DNA (ss-HSDNA/rGOae) modified on a rotating disk electrode (RDE) is presented. Owing to the large non-conductive biomolecule biding on the electrode, an electron transfer is interrupted and not easily accessible to a target molecule. To address this issue, we aim to study two effects; one considers electro-redox mediators and the other considers the hydrodynamic effect. By observing a cyclic voltammetric response from the ss-HSDNA/rGOae electrode in three different charges of the redox mediators (i.e., neutral FcCH2OH, cationic Ru(NH3)63+, and anionic Fe(CN)64-) in a phosphate buffer solution (PBS) containing AFB1, the magnitude of anodic current at 50 mV s-1 is 825, 615 and 550 mA cm-1, respectively which is significant dominated by the charge of the redox probe. The effect of hydrodynamic diffusion of the ss-HSDNA/rGOae rotating disk electrode (RDE) toward AFB1 detection using FcCH2OH as the redox mediator was recorded by applying a range of rotating speed from 500 to 4000 rpm. Increasing rotating speed reduces the charge transfer resistance resulting in the lower detectable level for AFB1 quantification. In the case of 4000 rpm, the AFB1 can be detected with a limit of detection of 0.04 ng/mL and a linear range of 1x10-10 to 7x10-8g/mL.
作者：Krittayavathananon, A；Sawangphruk, M
作者单位：Vidyasirimedhi Inst Sci & Technol
期刊名称：ANALYTICAL CHEMISTRY
期刊影响因子：5.886
出版年份：2017
出版刊次：24
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