中文摘要：本研究旨在比较用简单经济的定性方法（氨蒸气试验）和分析方法（薄层层析酶联免疫吸附试验）识别玉米中产毒黄曲霉菌株。研究发现培养法与分析法间具有良好的一致性，表明氨蒸气试验简单、经济、可靠而省时，可用于从散装食品/饲料库存中分离或预筛选受污染的样品。
外文摘要：An attempt was made to compare between easy and inexpensive qualitative method (ammonia vapour test) and analytical methods (thin layer chromatography and enzyme-linked immunosorbent assay) for identification of aflatoxigenic isolates of Aspergillus flavus in maize. In this comparative study the toxicity level of A. flavus isolates exhibited 100% agreement among ammonia vapour test, ELISA and TLC for highly toxigenic (>2000 ppb) and toxigenic (501-2000 ppb) isolates while 88.5% agreement observed for least toxic (<20 ppb) isolates. In ammonia vapour test 51% of A. flavus isolates showed creamish or no colour change corresponding to least toxic/atoxic (<20ppb) category estimated by ELISA. Similarly 22% highly toxic isolates exhibited plum red colour, 12% moderately toxic indicated pink colour and 10% toxic isolates showed red colour. However, 11.5% isolates were found to be false positive in cream colour category (least toxic) and 28.5% false negatives in pink colour (moderately toxic) category. The isolates from different agroclimatic zones of maize in India showed high variability for aflatoxin B1 (AFB1) production potential ranging from 0.214-8116.61 ppb. Toxigenic potential of Aspergillus flavus isolates in culture was further validated by inoculating maize grain sample with four different isolates with varied toxin producing ability. With good agreement percentage between cultural and analytical methods the study concludes the ammonia vapour test to be easy, inexpensive, reliable and time saving method that can be used for segregating or pre-screening of contaminated samples from bulk food/feed stock.
作者：Shekhar, M；Singh, N；Dutta, R；Kumar, S；Mahajan, V
作者单位：ICAR Res Complex
期刊名称：PLOS ONE
期刊影响因子：3.057
出版年份：2017
出版刊次：12
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