中文摘要：本研究研发了一种检测痕量黄曲霉毒素M1的电化学适体传感器。该传感器的动态范围为1–105ppt黄曲霉毒素M1。
外文摘要：An electrochemical aptasensor for detection of trace amounts of aflatoxin M1 was developed. This required immobilization of aptamer on screen printed gold electrode comprising of working electrode, counter electrode and reference electrode and was achieved by sequentially layering dithiodipropionic acid, streptavidin and biotinylated-tetraethylene glycol-aptamer. Immobilization of aptamer was monitored by cyclic voltammetry. Peak current in square wave voltammogram was inversely related to logarithmic concentration of aflatoxin M1. Dynamic range of sensor was 1–105 ppt aflatoxin M1. Sensor can be regenerated by treating electrode with 10% sodium dodecyl sulfate or 40 mM tris-HCl (pH 8.0) containing 10 mM ethylenediaminetetraacetic acid and 0.02% tween-20.
外文关键词：Electrochemical aptasensor；Aflatoxin M1；Aptamer；Square wave voltammetry
作者：Pandey, AK；Rajput, YS；Sharma, R；Singh, D
作者单位：NDRI
期刊名称：APPLIED NANOSCIENCE
期刊影响因子：3.325
出版年份：2017
出版刊次：8
点击下载：金电极固定化适体检测痕量黄曲霉毒素M1