中文摘要：转基因生物在进入市场前需要进行安全评估。迄今为止，在真核基因调控中发挥重要作用的miRNAs在目前的评估系统中没有得到考虑。本文用PCR和免疫带6种独立的BT和EPSPS转基因水稻，并利用实时定量PCR和免疫印迹分析了Cry1Ac和EPSPS的表达水平。此外，作者还利用深测序和生物信息学方法对6中转基因水稻和常规水稻发育种子中的miRNAs进行了研究。研究发现，尽管与常规水稻相比，转基因株系具有不同的整合位点、拷贝数和基因表达水平，且有21种差异表达的miRNAs。但转基因蛋白表达水平与miRNAs差异表达的数量无相关性。这项研究为转基因植物的miRNA组成提供了有用的数据，可能会对基于miRNA的转基因植物的风险评估有所帮助。
外文摘要：Safety assessment for genetically modified organisms (GMOs) is required before their release. To date, miRNAs that play important roles in eukaryotic gene regulation have not been considered in the current assessment system. In this study, we identified 6 independent Bt and EPSPS GM rice lines using PCR and immune strip. We analyzed the expression levels of Cry1Ac and EPSPS using quantitative real-time PCR and western blot. Further, miRNAs from the developing seeds of the 6 GM rice lines and the wild-type line were investigated using deep sequencing and bioinformatic approaches. Although these GM lines have different types of integration sites, copy numbers, and levels of gene expression, 21 differentially expressed miRNAs have been found compared to wild type. There is no correlation between transgenic protein expression level and the quantity of differentially expressed miRNAs. This study provides useful data about the miRNA composition of GM plants, and it might be helpful for future risk assessments of miRNA-based GM plants.
作者：Peng, Cheng; Chen, Xiaoyun; Wang, Xiaofu; 等.
作者单位：Zhejiang Acad Agr Sci
期刊名称：SCIENTIFIC REPORTS
期刊影响因子：4.259
出版年份：2018
出版刊次：1
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