中文摘要：棉铃虫是为害大量高商业价值的作物从而造成巨大经济损失的害虫。RNA干扰（RNAi）是控制害虫的有力工具，本文基于RNAi研发出了表达棉铃虫保幼激素酸甲基转移酶（HaJHAMT）基因dsRNA的转基因番茄。棉铃虫取食HaJHAMT转基因番茄，会导致其同源基因表达的严重（90%）下调，从而对其取食和变形产生不利影响。研究结果表明，由于昆虫的特异性及JH在昆虫生长和发育中的重要作用，JH下调是一个有力的目标；另外，利用RNAi技术可在作物抗虫性方面发挥重要作用。
外文摘要：Helicoverpa annigera is an important pest infesting a number of crops of high commercial value leading to huge economic losses globally. RNA interference (RNAi) is a potent tool for the control of insect pests and towards this objective, transgenic tomato expressing dsRNA of Juvenile hormone acid methyl transferase (JHAMT) gene of H. annigera was developed. The H. annigera larvae fed on HaJHAMT tomato leaves led to a severe (90%) down regulation of the cognate gene expression thus adversely affected the feeding and metamorphosis. Reduction in larval and pupal weight and inability to undergo complete metamorphosis were observed that led to larval-pupal intermediates and subsequently affected the eclosion. Thus, JH down regulation is an attractive target due to specificity to insects and its important role in insect growth and development to engineer insect resistance in crops using RNAi.
外文关键词：Helicoverpa annigera; Tomato; RNA interference; Juvenile hormone acid methyl transferase; Gene silencing; Insect resistance; Metamorphosis
作者：Prakash, Navale M.; Maligeppagol, Manamohan; Asokan, R.; 等.
作者单位：Indian Inst Hort Res
期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
期刊影响因子：0.0
出版年份：2018
出版刊次：2
点击下载：表达保幼激素酸甲基转移酶基因dsRNA的转基因番茄会影响棉铃虫幼虫的生长与发育