中文摘要：在环境中检测、监测和绘制转基因序列一直是生态学中长期存在的挑战。转基因DNA的真实映射需要一种稳健和恒温的DNA扩增方法，以便具有成本效益和现场特征。本文提出了一个具有这些特征的方法多重荧光法，该方法是将常用的CaMV启动子检测用于转基因植物以及α-玉米醇溶蛋白特异玉米，且其可以适用于任何其他的DNA序列。该方法采用环介导等温扩增（LAMP）的可视化实现具有DNA杂交探针的荧光淬灭剂系统。本文验证了改方法在转基因玉米、非转基因玉米和非转基因/非玉米物种中的适用性。
外文摘要：Detecting, monitoring, and mapping transgenic sequences in the environment has been a long-standing challenge in ecology. Given the level of social, economic, and scientific interest in them, transgenic (GM) DNA sequences stand as paradigmatic; such sequences further have the unique property of functioning as unequivocal markers in the environment, clearly showing the paths of gene flow between crops and their wild relatives, and across industrial and traditional forms of agriculture. True mapping of transgenic DNA at the landscape level requires a method of DNA amplification that is robust and isothermal in order to be cost effective and field-based. We present here a method with these characteristics. Our multiplex fluorescent method is for the detection of the p35s CaMV promoter frequently used in transgenic plants as well as the alpha-zein protein specific to maize, but can be applicable to any other DNA sequence. The method uses loop-mediated isothermal amplification (LAMP) with visualization achieved using a fluorophore-quencher system of DNA hybridizing probes. We demonstrate the applicability of this tri-color method to transgenic corn, non-transgenic corn, and non-transgenic/non-corn species.
外文关键词：LAMP; Isothermal DNA amplification; GMOs; Maize; Field detection; Multiplex LAMP; Fluorescent LAMP
作者：Bektas, Ali
作者单位：Univ Calif Berkeley
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.038
出版年份：2018
出版刊次：3
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