中文摘要：随着对油类安全问题的日益关注，急需研发一种简便而灵敏的方法来检测黄曲霉毒素B1（AFB1），一种花生油中常见的霉菌毒素。本研究分别用氨基末端AFB1适体(NH2-DNA1)、巯基末端AFB1互补适体(SH-DNA2)结合磁珠(CS-Fe3O4)作为增强纳米探针，AuNR@DNTB@Ag纳米棒(ADANRs)作报告探针，研发了表面增强拉曼散射（SERS）传感器，用来超灵敏检测AFB1。研究表明，用该SERS传感器检测AFB1，检测下限为 0.0036ng/mL，有效线性检测范围从0.01到100ng/mL，相关系数达到了0.986。此外，还通过检测两种霉菌毒素证明了WERS传感器的特异性，并采用标准加入回收试验进一步证实了该传感器检测实际的花生油中AFB1的准确性。
外文摘要：With growing concern on oil safety problems, developing a simple and sensitive method to detect Aflatoxin B1 (AFB1), a common mycotoxin in peanut oil, is very necessary. In this study, Surface-enhanced Raman Scattering (SERS) aptasensor was developed for ultrasensitive AFB1 detection using the amino-terminal AFB1 aptamer (NH2-DNA1); and thiol-terminal AFB1 complementary aptamer (SH-DNA2) conjugated magnetic-beads (CS-Fe3O4) as enrichment nanoprobe and AuNR@DNTB@Ag nanorods (ADANRs) as reporter nanoprobe respectively. 5,5'-Dithiobis(2-nitrobenzoicacid) (DNTB) with large Raman scattering cross-section and no fluorescence interference was embedded in Au and Ag core/shell nanorods as Raman reporter molecules. CS-Fe3O4 possessed excellent biocompatibility and superparamagnetism for rapid signal enrichment. Therefore, NH2-DNA1-CS-Fe3O4 and SH-DNA2-ADANRs were fabricated via the hybrid reaction between aptamers and complementary aptamers. When there is AFB1, AFB1 would competitively combine with the NH2-DNA1-CS-Fe3O4 inducing the dissociation of SH-DNA2-ADANRs from CS-Fe3O4 and further decreasing the SERS signal. Based on this developed SERS aptasensor, a low limit of 0.0036ng/mL and an effective linear detection range from 0.01 to 100ng/mL with the correlation coefficient up to 0.986 for AFB1 detection were obtained. Moreover, the specificity of this SERS aptasensor was demonstrated by detecting other two mycotoxins and its accuracy for AFB1 detection in real peanut oil was further confirmed by standard addition recovery test.
外文关键词：Aflatoxin B1；Aptasensor；SERS；CS-Fe3O4；AuNR@DNTB@Ag NRs
作者：Chen, QS；Yang, MX；Yang, XJ；Li, HH；Guo, ZM；Rahma, MH
作者单位：Jiangsu Univ
期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
期刊影响因子：2.653
出版年份：2018
出版刊次：1
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